TY - JOUR
T1 - 2-Deoxy-d-ribose-5-phosphate aldolase (DERA): applications and modifications
AU - Haridas, Meera
AU - Abdelraheem, Eman M.M.
AU - Hanefeld, Ulf
PY - 2018
Y1 - 2018
N2 - © 2018, The Author(s). 2-Deoxy-d-ribose-5-phosphate aldolase (DERA) is a class I aldolase that offers access to several building blocks for organic synthesis. It catalyzes the stereoselective C–C bond formation between acetaldehyde and numerous other aldehydes. However, the practical application of DERA as a biocatalyst is limited by its poor tolerance towards industrially relevant concentrations of aldehydes, in particular acetaldehyde. Therefore, the development of proper experimental conditions, including protein engineering and/or immobilization on appropriate supports, is required. The present review is aimed to provide a brief overview of DERA, its history, and progress made in understanding the functioning of the enzyme. Furthermore, the current understanding regarding aldehyde resistance of DERA and the various optimizations carried out to modify this property are discussed.
AB - © 2018, The Author(s). 2-Deoxy-d-ribose-5-phosphate aldolase (DERA) is a class I aldolase that offers access to several building blocks for organic synthesis. It catalyzes the stereoselective C–C bond formation between acetaldehyde and numerous other aldehydes. However, the practical application of DERA as a biocatalyst is limited by its poor tolerance towards industrially relevant concentrations of aldehydes, in particular acetaldehyde. Therefore, the development of proper experimental conditions, including protein engineering and/or immobilization on appropriate supports, is required. The present review is aimed to provide a brief overview of DERA, its history, and progress made in understanding the functioning of the enzyme. Furthermore, the current understanding regarding aldehyde resistance of DERA and the various optimizations carried out to modify this property are discussed.
KW - Aldol reaction
KW - Aldolase
KW - C–C bond
KW - DERA
KW - Immobilization
KW - Protein engineering
UR - http://www.scopus.com/inward/record.url?scp=85054546974&partnerID=8YFLogxK
U2 - 10.1007/s00253-018-9392-8
DO - 10.1007/s00253-018-9392-8
M3 - Article
AN - SCOPUS:85054546974
SN - 0175-7598
VL - 102
SP - 9959
EP - 9971
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 23
ER -