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3D Printing for the Fabrication of Biofilm-Based Functional Living Materials. / Balasubramanian, Srikkanth; Aubin-Tam, Marie Eve; Meyer, Anne S.

In: ACS Synthetic Biology, Vol. 8, No. 7, 2019, p. 1564-1567.

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Balasubramanian, Srikkanth ; Aubin-Tam, Marie Eve ; Meyer, Anne S. / 3D Printing for the Fabrication of Biofilm-Based Functional Living Materials. In: ACS Synthetic Biology. 2019 ; Vol. 8, No. 7. pp. 1564-1567.

BibTeX

@article{e7cb05fe15974058a90ba31be24439e1,
title = "3D Printing for the Fabrication of Biofilm-Based Functional Living Materials",
abstract = "Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to degradation of toxic chemicals. Biofilms exhibit high mechanical stiffness and are inherently tolerant to adverse conditions including the presence of antibiotics, pollutants, detergents, high temperature, changes in pH, etc. These features make biofilms resilient, which is beneficial for applications in dynamic environments such as bioleaching, bioremediation, materials production, and wastewater purification. We have recently described an easy and cost-effective method for 3D printing of bacteria and have extended this technology for 3D printing of genetically engineered Escherichia coli biofilms. Our 3D printing platform exploits simple alginate chemistry for printing of a bacteria-alginate bioink mixture onto calcium-containing agar surfaces, resulting in the formation of bacteria-encapsulating hydrogels with varying geometries. Bacteria in these hydrogels remain intact, spatially patterned, and viable for several days. Printing of engineered bacteria to produce inducible biofilms leads to formation of multilayered three-dimensional structures that can tolerate harsh chemical treatments. Synthetic biology and material science approaches provide the opportunity to append a wide range of useful functionalities to these 3D-printed biofilms. In this article, we describe the wide range of future applications possible for applying functional 3D-printed biofilms to the construction of living biofilm-derived materials in a large-scale and environmentally stable manner.",
keywords = "3D bioprinting, additive manufacturing, biofilms, material sciences, synthetic biology",
author = "Srikkanth Balasubramanian and Aubin-Tam, {Marie Eve} and Meyer, {Anne S.}",
note = "Accepted Author Manuscript",
year = "2019",
doi = "10.1021/acssynbio.9b00192",
language = "English",
volume = "8",
pages = "1564--1567",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "American Chemical Society (ACS)",
number = "7",

}

RIS

TY - JOUR

T1 - 3D Printing for the Fabrication of Biofilm-Based Functional Living Materials

AU - Balasubramanian, Srikkanth

AU - Aubin-Tam, Marie Eve

AU - Meyer, Anne S.

N1 - Accepted Author Manuscript

PY - 2019

Y1 - 2019

N2 - Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to degradation of toxic chemicals. Biofilms exhibit high mechanical stiffness and are inherently tolerant to adverse conditions including the presence of antibiotics, pollutants, detergents, high temperature, changes in pH, etc. These features make biofilms resilient, which is beneficial for applications in dynamic environments such as bioleaching, bioremediation, materials production, and wastewater purification. We have recently described an easy and cost-effective method for 3D printing of bacteria and have extended this technology for 3D printing of genetically engineered Escherichia coli biofilms. Our 3D printing platform exploits simple alginate chemistry for printing of a bacteria-alginate bioink mixture onto calcium-containing agar surfaces, resulting in the formation of bacteria-encapsulating hydrogels with varying geometries. Bacteria in these hydrogels remain intact, spatially patterned, and viable for several days. Printing of engineered bacteria to produce inducible biofilms leads to formation of multilayered three-dimensional structures that can tolerate harsh chemical treatments. Synthetic biology and material science approaches provide the opportunity to append a wide range of useful functionalities to these 3D-printed biofilms. In this article, we describe the wide range of future applications possible for applying functional 3D-printed biofilms to the construction of living biofilm-derived materials in a large-scale and environmentally stable manner.

AB - Bacterial biofilms are three-dimensional networks of cells entangled in a self-generated extracellular polymeric matrix composed of proteins, lipids, polysaccharides, and nucleic acids. Biofilms can establish themselves on virtually any accessible surface and lead to varying impacts ranging from infectious diseases to degradation of toxic chemicals. Biofilms exhibit high mechanical stiffness and are inherently tolerant to adverse conditions including the presence of antibiotics, pollutants, detergents, high temperature, changes in pH, etc. These features make biofilms resilient, which is beneficial for applications in dynamic environments such as bioleaching, bioremediation, materials production, and wastewater purification. We have recently described an easy and cost-effective method for 3D printing of bacteria and have extended this technology for 3D printing of genetically engineered Escherichia coli biofilms. Our 3D printing platform exploits simple alginate chemistry for printing of a bacteria-alginate bioink mixture onto calcium-containing agar surfaces, resulting in the formation of bacteria-encapsulating hydrogels with varying geometries. Bacteria in these hydrogels remain intact, spatially patterned, and viable for several days. Printing of engineered bacteria to produce inducible biofilms leads to formation of multilayered three-dimensional structures that can tolerate harsh chemical treatments. Synthetic biology and material science approaches provide the opportunity to append a wide range of useful functionalities to these 3D-printed biofilms. In this article, we describe the wide range of future applications possible for applying functional 3D-printed biofilms to the construction of living biofilm-derived materials in a large-scale and environmentally stable manner.

KW - 3D bioprinting

KW - additive manufacturing

KW - biofilms

KW - material sciences

KW - synthetic biology

UR - http://www.scopus.com/inward/record.url?scp=85070059565&partnerID=8YFLogxK

U2 - 10.1021/acssynbio.9b00192

DO - 10.1021/acssynbio.9b00192

M3 - Review article

VL - 8

SP - 1564

EP - 1567

JO - ACS Synthetic Biology

T2 - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 7

ER -

ID: 55737002