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A minimal system to establish microtubule-based cell polarity in vitro. / Taberner Carretero, Nuria.

2016. 173 p.

Research output: ThesisDissertation (TU Delft)Scientific

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@phdthesis{c87db99c45f84ee9bcf6c0667c2be618,
title = "A minimal system to establish microtubule-based cell polarity in vitro",
abstract = "In this thesis we took the challenge to in vitro reconstitute a minimal phenomenon essential for live: Cell polarity. This is an ubiquitous phenomenon that allows cells to define a direction for migration, growth or division. Our study focussed on microtubulebased establishment of polarity taking S. pombe as a model organism. In this rod-shaped unicellular organism, microtubules deposit polarity factor proteins to the poles of the cell, leaving only there cues for initiating the cascade for cellular growth. Such pattern formation is very robust and is believed to rely on feedback mechanisms. In our study, we hypothesized what would be the minimum components needed for the establishment of a polarized cortical pattern of proteins and developed an in vitro system that fulfils those requirements. Those where:• Microtubule-based transport of polarity factors.• Elongated cell shape.• Cortical receptor for the polarity factors.",
keywords = "Microtubules, +TIPS, Polarity, Reconstitution",
author = "{Taberner Carretero}, Nuria",
year = "2016",
doi = "10.4233/uuid:c87db99c-45f8-4ee9-bcf6-c0667c2be618",
language = "English",
isbn = "978-94-6299-446-1",

}

RIS

TY - THES

T1 - A minimal system to establish microtubule-based cell polarity in vitro

AU - Taberner Carretero, Nuria

PY - 2016

Y1 - 2016

N2 - In this thesis we took the challenge to in vitro reconstitute a minimal phenomenon essential for live: Cell polarity. This is an ubiquitous phenomenon that allows cells to define a direction for migration, growth or division. Our study focussed on microtubulebased establishment of polarity taking S. pombe as a model organism. In this rod-shaped unicellular organism, microtubules deposit polarity factor proteins to the poles of the cell, leaving only there cues for initiating the cascade for cellular growth. Such pattern formation is very robust and is believed to rely on feedback mechanisms. In our study, we hypothesized what would be the minimum components needed for the establishment of a polarized cortical pattern of proteins and developed an in vitro system that fulfils those requirements. Those where:• Microtubule-based transport of polarity factors.• Elongated cell shape.• Cortical receptor for the polarity factors.

AB - In this thesis we took the challenge to in vitro reconstitute a minimal phenomenon essential for live: Cell polarity. This is an ubiquitous phenomenon that allows cells to define a direction for migration, growth or division. Our study focussed on microtubulebased establishment of polarity taking S. pombe as a model organism. In this rod-shaped unicellular organism, microtubules deposit polarity factor proteins to the poles of the cell, leaving only there cues for initiating the cascade for cellular growth. Such pattern formation is very robust and is believed to rely on feedback mechanisms. In our study, we hypothesized what would be the minimum components needed for the establishment of a polarized cortical pattern of proteins and developed an in vitro system that fulfils those requirements. Those where:• Microtubule-based transport of polarity factors.• Elongated cell shape.• Cortical receptor for the polarity factors.

KW - Microtubules

KW - +TIPS

KW - Polarity

KW - Reconstitution

UR - http://resolver.tudelft.nl/uuid:c87db99c-45f8-4ee9-bcf6-c0667c2be618

U2 - 10.4233/uuid:c87db99c-45f8-4ee9-bcf6-c0667c2be618

DO - 10.4233/uuid:c87db99c-45f8-4ee9-bcf6-c0667c2be618

M3 - Dissertation (TU Delft)

SN - 978-94-6299-446-1

ER -

ID: 6655506