TY - JOUR
T1 - A Straightforward Approach for 3D Bacterial Printing
AU - Lehner, Benjamin A.E.
AU - Schmieden, Dominik T.
AU - Meyer, Anne S.
PY - 2017/7/21
Y1 - 2017/7/21
N2 - Sustainable and personally tailored materials production is an emerging challenge to society. Living organisms can produce and pattern an extraordinarily wide range of different molecules in a sustainable way. These natural systems offer an abundant source of inspiration for the development of new environmentally friendly materials production techniques. In this paper, we describe the first steps toward the 3-dimensional printing of bacterial cultures for materials production and patterning. This methodology combines the capability of bacteria to form new materials with the reproducibility and tailored approach of 3D printing systems. For this purpose, a commercial 3D printer was modified for bacterial systems, and new alginate-based bioink chemistry was developed. Printing temperature, printhead speed, and bioink extrusion rate were all adapted and customized to maximize bacterial health and spatial resolution of printed structures. Our combination of 3D printing technology with biological systems enables a sustainable approach for the production of numerous new materials.
AB - Sustainable and personally tailored materials production is an emerging challenge to society. Living organisms can produce and pattern an extraordinarily wide range of different molecules in a sustainable way. These natural systems offer an abundant source of inspiration for the development of new environmentally friendly materials production techniques. In this paper, we describe the first steps toward the 3-dimensional printing of bacterial cultures for materials production and patterning. This methodology combines the capability of bacteria to form new materials with the reproducibility and tailored approach of 3D printing systems. For this purpose, a commercial 3D printer was modified for bacterial systems, and new alginate-based bioink chemistry was developed. Printing temperature, printhead speed, and bioink extrusion rate were all adapted and customized to maximize bacterial health and spatial resolution of printed structures. Our combination of 3D printing technology with biological systems enables a sustainable approach for the production of numerous new materials.
KW - 3D printing
KW - alginate
KW - Escherichia coli
KW - synthetic biology
UR - http://resolver.tudelft.nl/uuid:c9511f73-4a86-46b6-9257-d9fd3e0dcf96
UR - http://www.scopus.com/inward/record.url?scp=85022326752&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.6b00395
DO - 10.1021/acssynbio.6b00395
M3 - Article
AN - SCOPUS:85022326752
SN - 2161-5063
VL - 6
SP - 1124
EP - 1130
JO - ACS Synthetic Biology
JF - ACS Synthetic Biology
IS - 7
ER -