Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

M. Quintavalla; P. Pozzi; Michel Verhaegen; Hielke Bijlsma; Hans Verstraete; S. Bonora

doi:10.1117/12.2290036

Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

M. Quintavalla, P. Pozzi, Michel Verhaegen, Hielke Bijlsma, Hans Verstraete, S. Bonora

Team Raf Van de Plas

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

2 Citations (Scopus)

80 Downloads (Pure)

Abstract

Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.

Original language	English
Title of host publication	Proceedings of SPIE
Subtitle of host publication	Multiphoton Microscopy in the Biomedical Sciences XVIII
Editors	A. Periasamy, P.T.C. So, K. König, X.S. Xie
Place of Publication	Bellingham, WA, USA
Publisher	SPIE
Number of pages	6
Volume	10498
ISBN (Electronic)	9781510614819
DOIs	https://doi.org/10.1117/12.2290036
Publication status	Published - 2018
Event	SPIE BiOS: Multiphoton Microscopy in the Biomedical Sciences XVIII 2018 - San Francisco, United States Duration: 28 Jan 2018 → 30 Jan 2018

Publication series

Name	Proceedings SPIE
Volume	10498

Conference

Conference	SPIE BiOS: Multiphoton Microscopy in the Biomedical Sciences XVIII 2018
Country/Territory	United States
City	San Francisco
Period	28/01/18 → 30/01/18

Keywords

Adaptive lens
Adaptive optics
High-resolution microscopy
optimizaion

Access to Document

10.1117/12.2290036

104981XFinal published version, 293 KB

Cite this

Quintavalla, M., Pozzi, P., Verhaegen, M., Bijlsma, H., Verstraete, H., & Bonora, S. (2018). Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens. In A. Periasamy, P. T. C. So, K. König, & X. S. Xie (Eds.), Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XVIII (Vol. 10498). Article 104981X (Proceedings SPIE; Vol. 10498). SPIE. https://doi.org/10.1117/12.2290036

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abstract = "Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.",

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Quintavalla, M, Pozzi, P, Verhaegen, M, Bijlsma, H, Verstraete, H & Bonora, S 2018, Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens. in A Periasamy, PTC So, K König & XS Xie (eds), Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XVIII. vol. 10498, 104981X, Proceedings SPIE, vol. 10498, SPIE, Bellingham, WA, USA, SPIE BiOS: Multiphoton Microscopy in the Biomedical Sciences XVIII 2018, San Francisco, California, United States, 28/01/18. https://doi.org/10.1117/12.2290036

Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens. / Quintavalla, M.; Pozzi, P.; Verhaegen, Michel et al.
Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XVIII. ed. / A. Periasamy; P.T.C. So; K. König; X.S. Xie. Vol. 10498 Bellingham, WA, USA: SPIE, 2018. 104981X (Proceedings SPIE; Vol. 10498).

Research output: Chapter in Book/Conference proceedings/Edited volume › Conference contribution › Scientific › peer-review

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AB - Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.

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Quintavalla M, Pozzi P, Verhaegen M, Bijlsma H, Verstraete H, Bonora S. Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens. In Periasamy A, So PTC, König K, Xie XS, editors, Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XVIII. Vol. 10498. Bellingham, WA, USA: SPIE. 2018. 104981X. (Proceedings SPIE). doi: 10.1117/12.2290036

Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

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