Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS

Ward van Helmond; Marcel de Puit

doi:10.1007/978-1-4939-9639-1_33

Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS

Ward van Helmond, Marcel de Puit^*

^*Corresponding author for this work

Research output: Chapter in Book/Conference proceedings/Edited volume › Chapter › Scientific › peer-review

1 Citation (Scopus)

Abstract

UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.

Original language	English
Title of host publication	AMINO ACID ANALYSIS, 2 EDITION
Editors	John M. Walker
Publisher	Springer
Pages	439-450
Volume	2030
ISBN (Electronic)	978-1-4939-9639-1-33
ISBN (Print)	978-1-4939-9638-4
DOIs	https://doi.org/10.1007/978-1-4939-9639-1_33
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	2030
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Amino acid analysis
Amino acid profiling
Fingerprint
HILIC
Mass spectrometry
UPLC-MS

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10.1007/978-1-4939-9639-1_33

Cite this

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keywords = "Amino acid analysis, Amino acid profiling, Fingerprint, HILIC, Mass spectrometry, UPLC-MS",

author = "{van Helmond}, Ward and {de Puit}, Marcel",

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KW - Amino acid analysis

KW - Amino acid profiling

KW - Fingerprint

KW - HILIC

KW - Mass spectrometry

KW - UPLC-MS

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BT - AMINO ACID ANALYSIS, 2 EDITION

A2 - Walker, John M.

PB - Springer

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Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS

Abstract

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