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Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components. / de Avila, Erica D.; Castro, Antonio G.B.; Tagit, Oya; Krom, Bastiaan P.; Löwik, Dennis; van Well, Ad A.; Bannenberg, Lars J.; Vergani, Carlos Eduardo; van den Beucken, Jeroen J.J.P.

In: Applied Surface Science, Vol. 488, 2019, p. 194-204.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

de Avila, ED, Castro, AGB, Tagit, O, Krom, BP, Löwik, D, van Well, AA, Bannenberg, LJ, Vergani, CE & van den Beucken, JJJP 2019, 'Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components', Applied Surface Science, vol. 488, pp. 194-204. https://doi.org/10.1016/j.apsusc.2019.05.154

APA

de Avila, E. D., Castro, A. G. B., Tagit, O., Krom, B. P., Löwik, D., van Well, A. A., Bannenberg, L. J., Vergani, C. E., & van den Beucken, J. J. J. P. (2019). Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components. Applied Surface Science, 488, 194-204. https://doi.org/10.1016/j.apsusc.2019.05.154

Vancouver

Author

de Avila, Erica D. ; Castro, Antonio G.B. ; Tagit, Oya ; Krom, Bastiaan P. ; Löwik, Dennis ; van Well, Ad A. ; Bannenberg, Lars J. ; Vergani, Carlos Eduardo ; van den Beucken, Jeroen J.J.P. / Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components. In: Applied Surface Science. 2019 ; Vol. 488. pp. 194-204.

BibTeX

@article{679194c5dd2e4e6ba8be5818c299f15f,
title = "Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components",
abstract = "Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region.",
keywords = "Anti-bacterial, Layer-by-layer, Porphyromonas gingivalis, Surface modification",
author = "{de Avila}, {Erica D.} and Castro, {Antonio G.B.} and Oya Tagit and Krom, {Bastiaan P.} and Dennis L{\"o}wik and {van Well}, {Ad A.} and Bannenberg, {Lars J.} and Vergani, {Carlos Eduardo} and {van den Beucken}, {Jeroen J.J.P.}",
year = "2019",
doi = "10.1016/j.apsusc.2019.05.154",
language = "English",
volume = "488",
pages = "194--204",
journal = "Applied Surface Science",
issn = "0169-4332",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Anti-bacterial efficacy via drug-delivery system from layer-by-layer coating for percutaneous dental implant components

AU - de Avila, Erica D.

AU - Castro, Antonio G.B.

AU - Tagit, Oya

AU - Krom, Bastiaan P.

AU - Löwik, Dennis

AU - van Well, Ad A.

AU - Bannenberg, Lars J.

AU - Vergani, Carlos Eduardo

AU - van den Beucken, Jeroen J.J.P.

PY - 2019

Y1 - 2019

N2 - Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region.

AB - Percutaneous medical devices are prone to bacterial contamination that causes dramatic clinical conditions. At the percutaneous level of dental implant systems, microbial pathogens induce biofilm formation that may result in bone resorption and dental implant loss. In view of peri-implantitis caused by bacterial inflammation at the percutaneous abutment region, we here establish a novel drug release system based on layer-by-layer (LbL)-deposited poly(acrylic acid) (PAA) and poly-L-lysine (PLL) coatings on titanium (Ti). Detailed multilayer coating characterization was performed by different microscopy and spectroscopy techniques to probe physical and chemical properties. Our data revealed a significant difference in roughness average between ten double layers coated (141 nm ±30) and uncoated Ti discs (115 nm ±40). Although roughness of the coatings increased significantly after immersion in water for 24 h at 37 °C, this physical property remained below 200 nm. Coating stability was confirmed under neutral and acidic pH, mimicking healthy and diseased/inflammatory environments, respectively. LbL coatings supported in vitro human keratinocytes growth, demonstrating absence of cytotoxic effects. Tetracycline (TC) showed an initial burst release under neutral and acidic conditions, which further demonstrated robust antibacterial efficacy against Porphyromonas gingivalis. However, a convenient pH-dependent 2-folds increase in TC release was observed for coatings incubated at pH = 4.5. Sustained TC release was observed from coatings up till 15 days of incubation in both pH conditions. These results demonstrate the potential application of this simple surface modification to leverage anti-bacterial efficacy at the percutaneous abutment region.

KW - Anti-bacterial

KW - Layer-by-layer

KW - Porphyromonas gingivalis

KW - Surface modification

UR - http://www.scopus.com/inward/record.url?scp=85066443452&partnerID=8YFLogxK

U2 - 10.1016/j.apsusc.2019.05.154

DO - 10.1016/j.apsusc.2019.05.154

M3 - Article

AN - SCOPUS:85066443452

VL - 488

SP - 194

EP - 204

JO - Applied Surface Science

JF - Applied Surface Science

SN - 0169-4332

ER -

ID: 54338524