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CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. / Aher, Amol; Kok, Maurits; Sharma, Ashwani; Rai, Ankit; Olieric, Natacha; Rodriguez-Garcia, Ruddi; Katrukha, Eugene A.; Weinert, Tobias; Olieric, Vincent; Kapitein, Lukas C.; Steinmetz, Michel O.; Dogterom, Marileen; Akhmanova, Anna.

In: Developmental Cell, Vol. 46, No. 1, 02.07.2018, p. 40-58.e8.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Aher, A, Kok, M, Sharma, A, Rai, A, Olieric, N, Rodriguez-Garcia, R, Katrukha, EA, Weinert, T, Olieric, V, Kapitein, LC, Steinmetz, MO, Dogterom, M & Akhmanova, A 2018, 'CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain' Developmental Cell, vol. 46, no. 1, pp. 40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032

APA

Aher, A., Kok, M., Sharma, A., Rai, A., Olieric, N., Rodriguez-Garcia, R., ... Akhmanova, A. (2018). CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Developmental Cell, 46(1), 40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032

Vancouver

Aher A, Kok M, Sharma A, Rai A, Olieric N, Rodriguez-Garcia R et al. CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. Developmental Cell. 2018 Jul 2;46(1):40-58.e8. https://doi.org/10.1016/j.devcel.2018.05.032

Author

Aher, Amol ; Kok, Maurits ; Sharma, Ashwani ; Rai, Ankit ; Olieric, Natacha ; Rodriguez-Garcia, Ruddi ; Katrukha, Eugene A. ; Weinert, Tobias ; Olieric, Vincent ; Kapitein, Lukas C. ; Steinmetz, Michel O. ; Dogterom, Marileen ; Akhmanova, Anna. / CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain. In: Developmental Cell. 2018 ; Vol. 46, No. 1. pp. 40-58.e8.

BibTeX

@article{d0f18e5bf6284c679bb332eab1ac4df5,
title = "CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain",
abstract = "The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening, termed catastrophes, including those induced by microtubule-destabilizing agents and physical barriers. Mammalian CLASPs encompass three TOG-like domains, TOG1, TOG2, and TOG3, none of which bind to free tubulin. TOG2 is essential for catastrophe suppression, whereas TOG3 mildly enhances rescues but cannot suppress catastrophes. These functions are inhibited by the C-terminal domain of CLASP2, while the TOG1 domain can release this auto-inhibition. TOG2 fused to a positively charged microtubule-binding peptide autonomously accumulates at growing but not shrinking ends, suppresses catastrophes, and stimulates rescues. CLASPs suppress catastrophes by stabilizing growing microtubule ends, including incomplete ones, preventing their depolymerization and promoting their recovery into complete tubes. TOG2 domain is the key determinant of these activities.",
keywords = "CLASP, CLIP-170, EB1, EB3, microfabricated barriers, microtubule dynamics, single-molecule biophysics, TOG domain, tubulin, X-ray crystallography",
author = "Amol Aher and Maurits Kok and Ashwani Sharma and Ankit Rai and Natacha Olieric and Ruddi Rodriguez-Garcia and Katrukha, {Eugene A.} and Tobias Weinert and Vincent Olieric and Kapitein, {Lukas C.} and Steinmetz, {Michel O.} and Marileen Dogterom and Anna Akhmanova",
year = "2018",
month = "7",
day = "2",
doi = "10.1016/j.devcel.2018.05.032",
language = "English",
volume = "46",
pages = "40--58.e8",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - CLASP Suppresses Microtubule Catastrophes through a Single TOG Domain

AU - Aher, Amol

AU - Kok, Maurits

AU - Sharma, Ashwani

AU - Rai, Ankit

AU - Olieric, Natacha

AU - Rodriguez-Garcia, Ruddi

AU - Katrukha, Eugene A.

AU - Weinert, Tobias

AU - Olieric, Vincent

AU - Kapitein, Lukas C.

AU - Steinmetz, Michel O.

AU - Dogterom, Marileen

AU - Akhmanova, Anna

PY - 2018/7/2

Y1 - 2018/7/2

N2 - The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening, termed catastrophes, including those induced by microtubule-destabilizing agents and physical barriers. Mammalian CLASPs encompass three TOG-like domains, TOG1, TOG2, and TOG3, none of which bind to free tubulin. TOG2 is essential for catastrophe suppression, whereas TOG3 mildly enhances rescues but cannot suppress catastrophes. These functions are inhibited by the C-terminal domain of CLASP2, while the TOG1 domain can release this auto-inhibition. TOG2 fused to a positively charged microtubule-binding peptide autonomously accumulates at growing but not shrinking ends, suppresses catastrophes, and stimulates rescues. CLASPs suppress catastrophes by stabilizing growing microtubule ends, including incomplete ones, preventing their depolymerization and promoting their recovery into complete tubes. TOG2 domain is the key determinant of these activities.

AB - The dynamic instability of microtubules plays a key role in controlling their organization and function, but the cellular mechanisms regulating this process are poorly understood. Here, we show that cytoplasmic linker-associated proteins (CLASPs) suppress transitions from microtubule growth to shortening, termed catastrophes, including those induced by microtubule-destabilizing agents and physical barriers. Mammalian CLASPs encompass three TOG-like domains, TOG1, TOG2, and TOG3, none of which bind to free tubulin. TOG2 is essential for catastrophe suppression, whereas TOG3 mildly enhances rescues but cannot suppress catastrophes. These functions are inhibited by the C-terminal domain of CLASP2, while the TOG1 domain can release this auto-inhibition. TOG2 fused to a positively charged microtubule-binding peptide autonomously accumulates at growing but not shrinking ends, suppresses catastrophes, and stimulates rescues. CLASPs suppress catastrophes by stabilizing growing microtubule ends, including incomplete ones, preventing their depolymerization and promoting their recovery into complete tubes. TOG2 domain is the key determinant of these activities.

KW - CLASP

KW - CLIP-170

KW - EB1

KW - EB3

KW - microfabricated barriers

KW - microtubule dynamics

KW - single-molecule biophysics

KW - TOG domain

KW - tubulin

KW - X-ray crystallography

UR - http://www.scopus.com/inward/record.url?scp=85048562628&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2018.05.032

DO - 10.1016/j.devcel.2018.05.032

M3 - Article

VL - 46

SP - 40-58.e8

JO - Developmental Cell

T2 - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 1

ER -

ID: 45664939