Collection of amino acids and DNA from fingerprints using hydrogels

Ward Van Helmond; Vincent O'Brien; Robin De Jong; Jan Van Esch; Sander Oldenhof; Marcel De Puit

doi:10.1039/c7an01692a

Collection of amino acids and DNA from fingerprints using hydrogels

Ward Van Helmond, Vincent O'Brien, Robin De Jong, Jan Van Esch, Sander Oldenhof^*, Marcel De Puit

^*Corresponding author for this work

ChemE/Advanced Soft Matter

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

41 Downloads (Pure)

Abstract

The amino acid profile obtained from a fingerprint may provide valuable information on its donor. For forensic scientists, recovering evidence relating to the amino acid profile of a suspect can potentially be valuable for identification and exclusion purposes. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualisation at a later stage using the standard method of cyanoacrylation. We will demonstrate this method to also be capable of collecting DNA from fingerprints with a 20-60% yield in comparison to using a conventional cotton swab.

Original language	English
Pages (from-to)	900-905
Number of pages	6
Journal	Analyst
Volume	143
Issue number	4
DOIs	https://doi.org/10.1039/c7an01692a
Publication status	Published - 21 Feb 2018

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c7an01692aFinal published version, 1.75 MBLicence: CC BY-NC

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AU - O'Brien, Vincent

AU - De Jong, Robin

AU - Van Esch, Jan

AU - Oldenhof, Sander

AU - De Puit, Marcel

PY - 2018/2/21

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Collection of amino acids and DNA from fingerprints using hydrogels

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