Confocal Multi-line Scanning Microscope for Efficient 3D Fluorescence Imaging

Leon van der Graaff, Sjoerd Stallinga

Research output: Chapter in Book/Conference proceedings/Edited volumeConference contributionScientificpeer-review

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Abstract

Confocal fluorescent imaging is the de facto standard modality for fluorescence imaging. However, the point-to-point scanning technique leads to a very limited throughput and makes the technique unsuitable for large area and fast multi-focal scanning. We propose an architecture for highly efficient 3D line confocal fluorescence imaging. Our design extends the concept of a line scanning system with continuous ‘push broom’ scanning. Instead of using a line sensor, we use an area sensor that is tilted with respect to the optical axis to acquire image data of multiple depths simultaneously. A multi-line illumination with lines illuminating the specimen at different depths, conjugate to the tilted area sensor, is created by means of a diffractive optical element (DOE). The proposed method is suitable for fast 3D image acquisition with unlimited field of view, it requires no moving components except for the sample scanning stage, has intrinsically low losses, and provides intrinsic alignment of the simultaneously scanned layers of the focal stack.
Original languageEnglish
Title of host publicationApplied Industrial Optics
Subtitle of host publicationSpectroscopy, Imaging and Metrology - Proceedings Applied Industrial Optics 2019
PublisherOSA Publishing
Number of pages2
ISBN (Electronic)978-1-943580-65-1
DOIs
Publication statusPublished - 2019
EventApplied Industrial Optics: Spectroscopy, Imaging and Metrology 2019 - Washington, DC, United States
Duration: 8 Jul 201910 Jul 2019

Publication series

NameApplied Industrial Optics: Spectroscopy, Imaging and Metrology - Proceedings Applied Industrial Optics 2019

Conference

ConferenceApplied Industrial Optics: Spectroscopy, Imaging and Metrology 2019
Country/TerritoryUnited States
CityWashington, DC
Period8/07/1910/07/19

Bibliographical note

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

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