TY - GEN
T1 - Confocal Multi-line Scanning Microscope for Efficient 3D Fluorescence Imaging
AU - van der Graaff, Leon
AU - Stallinga, Sjoerd
N1 - Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
PY - 2019
Y1 - 2019
N2 - Confocal fluorescent imaging is the de facto standard modality for fluorescence imaging. However, the point-to-point scanning technique leads to a very limited throughput and makes the technique unsuitable for large area and fast multi-focal scanning. We propose an architecture for highly efficient 3D line confocal fluorescence imaging. Our design extends the concept of a line scanning system with continuous ‘push broom’ scanning. Instead of using a line sensor, we use an area sensor that is tilted with respect to the optical axis to acquire image data of multiple depths simultaneously. A multi-line illumination with lines illuminating the specimen at different depths, conjugate to the tilted area sensor, is created by means of a diffractive optical element (DOE). The proposed method is suitable for fast 3D image acquisition with unlimited field of view, it requires no moving components except for the sample scanning stage, has intrinsically low losses, and provides intrinsic alignment of the simultaneously scanned layers of the focal stack.
AB - Confocal fluorescent imaging is the de facto standard modality for fluorescence imaging. However, the point-to-point scanning technique leads to a very limited throughput and makes the technique unsuitable for large area and fast multi-focal scanning. We propose an architecture for highly efficient 3D line confocal fluorescence imaging. Our design extends the concept of a line scanning system with continuous ‘push broom’ scanning. Instead of using a line sensor, we use an area sensor that is tilted with respect to the optical axis to acquire image data of multiple depths simultaneously. A multi-line illumination with lines illuminating the specimen at different depths, conjugate to the tilted area sensor, is created by means of a diffractive optical element (DOE). The proposed method is suitable for fast 3D image acquisition with unlimited field of view, it requires no moving components except for the sample scanning stage, has intrinsically low losses, and provides intrinsic alignment of the simultaneously scanned layers of the focal stack.
UR - http://www.scopus.com/inward/record.url?scp=85085644321&partnerID=8YFLogxK
U2 - 10.1364/AIO.2019.W2A.5
DO - 10.1364/AIO.2019.W2A.5
M3 - Conference contribution
T3 - Applied Industrial Optics: Spectroscopy, Imaging and Metrology - Proceedings Applied Industrial Optics 2019
BT - Applied Industrial Optics
PB - OSA Publishing
T2 - Applied Industrial Optics: Spectroscopy, Imaging and Metrology 2019
Y2 - 8 July 2019 through 10 July 2019
ER -