Abstract
Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.
Original language | English |
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Pages (from-to) | 39-50.e10 |
Journal | Molecular Cell |
Volume | 77 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2020 |
Keywords
- arms race
- Cascade
- CRISPR-Cas
- PALM
- PAM
- photo-activation localization microscopy
- single molecule
- single-particle tracking
- target search