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FtsZ-Induced Shape Transformation of Coacervates. / Fanalista, Federico; Deshpande, Siddharth; Lau, Anson; Pawlik, Grzegorz; Dekker, Cees.

In: ADVANCED BIOSYSTEMS, Vol. 2, No. 9, 2018.

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@article{d4f2d6095c594b0d8a4fda0297d8fad5,
title = "FtsZ-Induced Shape Transformation of Coacervates",
abstract = "Recently, both the cellular and synthetic biology communities have expressed a strong interest in coacervates, membrane‐less liquid droplets composed of densely packed multivalent molecules that form as a result of spontaneous phase separation. Here, it is studied how FtsZ, a protein that plays a key role in the bacterial division process, remodels coacervates made of polylysine (pLL) and guanosine triphosphate (GTP). It is shown that FtsZ strongly partitions at the surface of the coacervates and induces their disassembly due to the hydrolysis of GTP by FtsZ. Surprisingly, the coacervates are found to promote lateral interactions between FtsZ filaments, inducing the formation of an emanating network of FtsZ bundles that interconnect neighboring coacervates. Under mechanical stress, coacervates are shown to fracture, resulting in profound invaginations along their circumference. The results bring out the potential of coacervates for their use as membrane‐free scaffolds for building synthetic cells as well as are possibly relevant for coacervation in prokaryotic cells.",
keywords = "biointerface, coacervates, FtsZ, protein bundling, synthetic cell",
author = "Federico Fanalista and Siddharth Deshpande and Anson Lau and Grzegorz Pawlik and Cees Dekker",
year = "2018",
doi = "10.1002/adbi.201800136",
language = "English",
volume = "2",
journal = "ADVANCED BIOSYSTEMS",
issn = "2366-7478",
publisher = "Wiley-VCH",
number = "9",

}

RIS

TY - JOUR

T1 - FtsZ-Induced Shape Transformation of Coacervates

AU - Fanalista, Federico

AU - Deshpande, Siddharth

AU - Lau, Anson

AU - Pawlik, Grzegorz

AU - Dekker, Cees

PY - 2018

Y1 - 2018

N2 - Recently, both the cellular and synthetic biology communities have expressed a strong interest in coacervates, membrane‐less liquid droplets composed of densely packed multivalent molecules that form as a result of spontaneous phase separation. Here, it is studied how FtsZ, a protein that plays a key role in the bacterial division process, remodels coacervates made of polylysine (pLL) and guanosine triphosphate (GTP). It is shown that FtsZ strongly partitions at the surface of the coacervates and induces their disassembly due to the hydrolysis of GTP by FtsZ. Surprisingly, the coacervates are found to promote lateral interactions between FtsZ filaments, inducing the formation of an emanating network of FtsZ bundles that interconnect neighboring coacervates. Under mechanical stress, coacervates are shown to fracture, resulting in profound invaginations along their circumference. The results bring out the potential of coacervates for their use as membrane‐free scaffolds for building synthetic cells as well as are possibly relevant for coacervation in prokaryotic cells.

AB - Recently, both the cellular and synthetic biology communities have expressed a strong interest in coacervates, membrane‐less liquid droplets composed of densely packed multivalent molecules that form as a result of spontaneous phase separation. Here, it is studied how FtsZ, a protein that plays a key role in the bacterial division process, remodels coacervates made of polylysine (pLL) and guanosine triphosphate (GTP). It is shown that FtsZ strongly partitions at the surface of the coacervates and induces their disassembly due to the hydrolysis of GTP by FtsZ. Surprisingly, the coacervates are found to promote lateral interactions between FtsZ filaments, inducing the formation of an emanating network of FtsZ bundles that interconnect neighboring coacervates. Under mechanical stress, coacervates are shown to fracture, resulting in profound invaginations along their circumference. The results bring out the potential of coacervates for their use as membrane‐free scaffolds for building synthetic cells as well as are possibly relevant for coacervation in prokaryotic cells.

KW - biointerface

KW - coacervates

KW - FtsZ

KW - protein bundling

KW - synthetic cell

U2 - 10.1002/adbi.201800136

DO - 10.1002/adbi.201800136

M3 - Article

VL - 2

JO - ADVANCED BIOSYSTEMS

T2 - ADVANCED BIOSYSTEMS

JF - ADVANCED BIOSYSTEMS

SN - 2366-7478

IS - 9

ER -

ID: 47329282