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Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus. / van Schie, B.J. ; Pronk, Jack; Hellingwerf, K.J.; van Dijken, JP; Kuenen, J.G.

In: Microbiology, Vol. 133, No. 12, 1987, p. 3427-3435.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

van Schie, BJ, Pronk, J, Hellingwerf, KJ, van Dijken, JP & Kuenen, JG 1987, 'Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus' Microbiology, vol. 133, no. 12, pp. 3427-3435. https://doi.org/10.1099/00221287-133-12-3427

APA

van Schie, B. J., Pronk, J., Hellingwerf, K. J., van Dijken, JP., & Kuenen, J. G. (1987). Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus. Microbiology, 133(12), 3427-3435. https://doi.org/10.1099/00221287-133-12-3427

Vancouver

Author

van Schie, B.J. ; Pronk, Jack ; Hellingwerf, K.J. ; van Dijken, JP ; Kuenen, J.G. / Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus. In: Microbiology. 1987 ; Vol. 133, No. 12. pp. 3427-3435.

BibTeX

@article{6f330d82369e419eb813069676844453,
title = "Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus",
abstract = "Evidence is presented that in Acinetobacter calcoaceticus oxidation of glucose to gluconate by the periplasmic quinoprotein glucose dehydrogenase (EC 1 . 1 .99.17) leads to energy conservation. Membrane vesicles prepared from cells grown in carbon-limited chemostat culture exhibited (1) a high rate of glucose-dependent oxygen consumption and gluconate production, (2) glucosemediated cytochrome reduction, (3) uncoupler sensitive, glucose-dependent generation of a membrane potential and (4) glucose-driven accumulation of amino acids. Furthermore, oxidation of glucose to gluconate by whole cells was associated with ATP synthesis. These results confirm and extend previous observations that periplasmic glucose oxidation can act as a driving force for energy-requiring processes. It is therefore concluded that the incomplete oxidation of glucose by bacteria may serve as an auxiliary energy-generating system.",
author = "{van Schie}, B.J. and Jack Pronk and K.J. Hellingwerf and {van Dijken}, JP and J.G. Kuenen",
year = "1987",
doi = "10.1099/00221287-133-12-3427",
language = "English",
volume = "133",
pages = "3427--3435",
journal = "Microbiology",
issn = "1350-0872",
publisher = "Society for General Microbiology",
number = "12",

}

RIS

TY - JOUR

T1 - Glucose-dehydrogenase-mediated solute transport and ATP synthesis in Acinetobacter calcoaceticus

AU - van Schie, B.J.

AU - Pronk, Jack

AU - Hellingwerf, K.J.

AU - van Dijken, JP

AU - Kuenen, J.G.

PY - 1987

Y1 - 1987

N2 - Evidence is presented that in Acinetobacter calcoaceticus oxidation of glucose to gluconate by the periplasmic quinoprotein glucose dehydrogenase (EC 1 . 1 .99.17) leads to energy conservation. Membrane vesicles prepared from cells grown in carbon-limited chemostat culture exhibited (1) a high rate of glucose-dependent oxygen consumption and gluconate production, (2) glucosemediated cytochrome reduction, (3) uncoupler sensitive, glucose-dependent generation of a membrane potential and (4) glucose-driven accumulation of amino acids. Furthermore, oxidation of glucose to gluconate by whole cells was associated with ATP synthesis. These results confirm and extend previous observations that periplasmic glucose oxidation can act as a driving force for energy-requiring processes. It is therefore concluded that the incomplete oxidation of glucose by bacteria may serve as an auxiliary energy-generating system.

AB - Evidence is presented that in Acinetobacter calcoaceticus oxidation of glucose to gluconate by the periplasmic quinoprotein glucose dehydrogenase (EC 1 . 1 .99.17) leads to energy conservation. Membrane vesicles prepared from cells grown in carbon-limited chemostat culture exhibited (1) a high rate of glucose-dependent oxygen consumption and gluconate production, (2) glucosemediated cytochrome reduction, (3) uncoupler sensitive, glucose-dependent generation of a membrane potential and (4) glucose-driven accumulation of amino acids. Furthermore, oxidation of glucose to gluconate by whole cells was associated with ATP synthesis. These results confirm and extend previous observations that periplasmic glucose oxidation can act as a driving force for energy-requiring processes. It is therefore concluded that the incomplete oxidation of glucose by bacteria may serve as an auxiliary energy-generating system.

U2 - 10.1099/00221287-133-12-3427

DO - 10.1099/00221287-133-12-3427

M3 - Article

VL - 133

SP - 3427

EP - 3435

JO - Microbiology

T2 - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 12

ER -

ID: 66960435