TY - JOUR
T1 - Handling and Sensing of Single Enzyme Molecules
T2 - From Fluorescence Detection towards Nanoscale Electrical Measurements
AU - Mathwig, Klaus
AU - Chi, Qijin
AU - Lemay, Serge G.
AU - Rassaei, Liza
PY - 2016
Y1 - 2016
N2 - Classical methods to study single enzyme molecules have provided valuable information about the distribution of conformational heterogeneities, reaction mechanisms, and transients in enzymatic reactions when individual molecules instead of an averaging ensemble are studied. Here, we highlight major advances in all-electrical single enzyme studies with a focus on recent micro- and nanofluidic tools, which offer new ways of handling and studying small numbers of molecules or even single enzyme molecules. We particularly emphasize nanofluidic devices, which enable the integration of electrochemical transduction and detection.
AB - Classical methods to study single enzyme molecules have provided valuable information about the distribution of conformational heterogeneities, reaction mechanisms, and transients in enzymatic reactions when individual molecules instead of an averaging ensemble are studied. Here, we highlight major advances in all-electrical single enzyme studies with a focus on recent micro- and nanofluidic tools, which offer new ways of handling and studying small numbers of molecules or even single enzyme molecules. We particularly emphasize nanofluidic devices, which enable the integration of electrochemical transduction and detection.
KW - electrochemical sensing
KW - microdroplets
KW - nanofluidics
KW - single enzymes
KW - single molecules
UR - http://www.scopus.com/inward/record.url?scp=84958861624&partnerID=8YFLogxK
U2 - 10.1002/cphc.201500686
DO - 10.1002/cphc.201500686
M3 - Article
C2 - 26458730
AN - SCOPUS:84958861624
SN - 1439-4235
VL - 17
SP - 452
EP - 457
JO - ChemPhysChem: a European journal of chemical physics and physical chemistry
JF - ChemPhysChem: a European journal of chemical physics and physical chemistry
IS - 4
ER -