TY - JOUR
T1 - Human osteoblast-derived extracellular matrix with high homology to bone proteome is osteopromotive
AU - Baroncelli, Marta
AU - Van Der Eerden, Bram C.J.
AU - Chatterji, Siddharth
AU - Rull Trinidad, Enrique
AU - Kan, Yik Y.
AU - Koedam, Marijke
AU - Van Hengel, Ingmar A.J.
AU - Alves, Rodrigo D.A.M.
AU - Fratila-Apachitei, Lidy E.
AU - Demmers, Jeroen A.A.
AU - Van De Peppel, Jeroen
AU - Van Leeuwen, Johannes P.T.M.
PY - 2018
Y1 - 2018
N2 - Efficient osteogenic differentiation of mesenchymal stromal cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation. Osteogenic differentiation and mineralization of MSCs on the ECM were quicker than in standard conditions. The presence of ECM promoted in vivo bone formation by MSCs in a mouse model of ectopic calcification. We analyzed the ECM composition by mass spectrometry, detecting 846 proteins. Of these, 473 proteins were shared with the human bone proteome we previously described, demonstrating high homology to an in vivo microenvironment. Bioinformatic analysis of the 846 proteins showed involvement in adhesion and osteogenic differentiation, confirming the ECM composition as key modulator of MSC behavior. In addition to known ECM components, proteomic analysis revealed novel ECM functions, which could improve culture conditions. In summary, this study provides a simplified method to obtain an in vitro MSC-derived ECM that enhances osteogenic differentiation and could be applied as natural biomaterial to accelerate bone regeneration.
AB - Efficient osteogenic differentiation of mesenchymal stromal cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation. Osteogenic differentiation and mineralization of MSCs on the ECM were quicker than in standard conditions. The presence of ECM promoted in vivo bone formation by MSCs in a mouse model of ectopic calcification. We analyzed the ECM composition by mass spectrometry, detecting 846 proteins. Of these, 473 proteins were shared with the human bone proteome we previously described, demonstrating high homology to an in vivo microenvironment. Bioinformatic analysis of the 846 proteins showed involvement in adhesion and osteogenic differentiation, confirming the ECM composition as key modulator of MSC behavior. In addition to known ECM components, proteomic analysis revealed novel ECM functions, which could improve culture conditions. In summary, this study provides a simplified method to obtain an in vitro MSC-derived ECM that enhances osteogenic differentiation and could be applied as natural biomaterial to accelerate bone regeneration.
KW - bone
KW - bone tissue engineering
KW - extracellular matrix
KW - mesenchymal stromal cells
UR - http://www.scopus.com/inward/record.url?scp=85052926747&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2017.0448
DO - 10.1089/ten.tea.2017.0448
M3 - Article
AN - SCOPUS:85052926747
SN - 1937-3341
VL - 24
SP - 1377
EP - 1389
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
IS - 17-18
ER -