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Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material. / Appels, Freek V. W.; Dijksterhuis, Jan; Lukasiewicz, Catherine E.; Jansen, Kaspar; Wösten, Han A.B.; Krijgsheld, Pauline.

In: Scientific Reports, Vol. 8, No. 1, 4703, 2018, p. 1-7.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Appels, FVW, Dijksterhuis, J, Lukasiewicz, CE, Jansen, K, Wösten, HAB & Krijgsheld, P 2018, 'Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material' Scientific Reports, vol. 8, no. 1, 4703, pp. 1-7. https://doi.org/10.1038/s41598-018-23171-2

APA

Appels, F. V. W., Dijksterhuis, J., Lukasiewicz, C. E., Jansen, K., Wösten, H. A. B., & Krijgsheld, P. (2018). Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material. Scientific Reports, 8(1), 1-7. [4703]. https://doi.org/10.1038/s41598-018-23171-2

Vancouver

Author

Appels, Freek V. W. ; Dijksterhuis, Jan ; Lukasiewicz, Catherine E. ; Jansen, Kaspar ; Wösten, Han A.B. ; Krijgsheld, Pauline. / Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material. In: Scientific Reports. 2018 ; Vol. 8, No. 1. pp. 1-7.

BibTeX

@article{8265668f6994409fbf1786307d135b01,
title = "Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material",
abstract = "Filamentous fungi colonize substrates by forming a mycelium. This network of hyphae can be used as a bio-based material. Here, we assessed the impact of environmental growth conditions and deletion of the hydrophobin gene sc3 on material properties of the mycelium of the mushroom forming fungus Schizophyllum commune. Thermogravimetric analysis showed that Δsc3 mycelium retained more water with increasing temperature when compared to the wild type. The Young's modulus (E) of the mycelium ranged between 438 and 913 MPa when the wild type strain was grown in the dark or in the light at low or high CO2 levels. This was accompanied by a maximum tensile strength (σ) of 5.1-9.6 MPa. In contrast, E and σ of the Δsc3 strain were 3-4- fold higher with values of 1237-2727 MPa and 15.6-40.4 MPa, respectively. These values correlated with mycelium density, while no differences in chemical composition of the mycelia were observed as shown by ATR-FTIR. Together, genetic modification and environmental growth conditions impact mechanical properties of the mycelium by affecting the density of the mycelium. As a result, mechanical properties of wild type mycelium were similar to those of natural materials, while those of Δsc3 were more similar to thermoplastics.",
author = "Appels, {Freek V. W.} and Jan Dijksterhuis and Lukasiewicz, {Catherine E.} and Kaspar Jansen and W{\"o}sten, {Han A.B.} and Pauline Krijgsheld",
year = "2018",
doi = "10.1038/s41598-018-23171-2",
language = "English",
volume = "8",
pages = "1--7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Hydrophobin gene deletion and environmental growth conditions impact mechanical properties of mycelium by affecting the density of the material

AU - Appels, Freek V. W.

AU - Dijksterhuis, Jan

AU - Lukasiewicz, Catherine E.

AU - Jansen, Kaspar

AU - Wösten, Han A.B.

AU - Krijgsheld, Pauline

PY - 2018

Y1 - 2018

N2 - Filamentous fungi colonize substrates by forming a mycelium. This network of hyphae can be used as a bio-based material. Here, we assessed the impact of environmental growth conditions and deletion of the hydrophobin gene sc3 on material properties of the mycelium of the mushroom forming fungus Schizophyllum commune. Thermogravimetric analysis showed that Δsc3 mycelium retained more water with increasing temperature when compared to the wild type. The Young's modulus (E) of the mycelium ranged between 438 and 913 MPa when the wild type strain was grown in the dark or in the light at low or high CO2 levels. This was accompanied by a maximum tensile strength (σ) of 5.1-9.6 MPa. In contrast, E and σ of the Δsc3 strain were 3-4- fold higher with values of 1237-2727 MPa and 15.6-40.4 MPa, respectively. These values correlated with mycelium density, while no differences in chemical composition of the mycelia were observed as shown by ATR-FTIR. Together, genetic modification and environmental growth conditions impact mechanical properties of the mycelium by affecting the density of the mycelium. As a result, mechanical properties of wild type mycelium were similar to those of natural materials, while those of Δsc3 were more similar to thermoplastics.

AB - Filamentous fungi colonize substrates by forming a mycelium. This network of hyphae can be used as a bio-based material. Here, we assessed the impact of environmental growth conditions and deletion of the hydrophobin gene sc3 on material properties of the mycelium of the mushroom forming fungus Schizophyllum commune. Thermogravimetric analysis showed that Δsc3 mycelium retained more water with increasing temperature when compared to the wild type. The Young's modulus (E) of the mycelium ranged between 438 and 913 MPa when the wild type strain was grown in the dark or in the light at low or high CO2 levels. This was accompanied by a maximum tensile strength (σ) of 5.1-9.6 MPa. In contrast, E and σ of the Δsc3 strain were 3-4- fold higher with values of 1237-2727 MPa and 15.6-40.4 MPa, respectively. These values correlated with mycelium density, while no differences in chemical composition of the mycelia were observed as shown by ATR-FTIR. Together, genetic modification and environmental growth conditions impact mechanical properties of the mycelium by affecting the density of the mycelium. As a result, mechanical properties of wild type mycelium were similar to those of natural materials, while those of Δsc3 were more similar to thermoplastics.

UR - http://www.scopus.com/inward/record.url?scp=85044268095&partnerID=8YFLogxK

UR - http://resolver.tudelft.nl/uuid:8265668f-6994-409f-bf17-86307d135b01

U2 - 10.1038/s41598-018-23171-2

DO - 10.1038/s41598-018-23171-2

M3 - Article

VL - 8

SP - 1

EP - 7

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 4703

ER -

ID: 43038616