TY - JOUR
T1 - Investigating biofilm structure developing on carriers from lab-scale moving bed biofilm reactors based on light microscopy and optical coherence tomography
AU - Li, Chunyan
AU - Felz, Simon
AU - Wagner, Michael
AU - Lackner, Susanne
AU - Horn, Harald
PY - 2016
Y1 - 2016
N2 - This study focused on characterizing the structure of biofilms developed on carriers used in lab-scale moving bed biofilm reactors. Both light microscopy (2D) and optical coherence tomography (OCT) were employed to track the biofilm development on carriers of different geometry and under different aeration rates. Biofilm structure was further characterized with respect to average biofilm thickness, biofilm growth velocity, biomass volume, compartment filling degree, surface area, etc. The results showed that carriers with a smaller compartment size stimulated a quick establishment of biofilms. Low aeration rates favored fast development of biofilms. Comparison between the results derived from 2D and 3D images revealed comparable results with respect to average biofilm thickness and compartment filling degree before the carrier compartments were fully willed with biomass. However, 3D imaging with OCT was capable of visualizing and quantifying the heterogeneous structure of biofilms, which cannot be achieved using 2D imaging.
AB - This study focused on characterizing the structure of biofilms developed on carriers used in lab-scale moving bed biofilm reactors. Both light microscopy (2D) and optical coherence tomography (OCT) were employed to track the biofilm development on carriers of different geometry and under different aeration rates. Biofilm structure was further characterized with respect to average biofilm thickness, biofilm growth velocity, biomass volume, compartment filling degree, surface area, etc. The results showed that carriers with a smaller compartment size stimulated a quick establishment of biofilms. Low aeration rates favored fast development of biofilms. Comparison between the results derived from 2D and 3D images revealed comparable results with respect to average biofilm thickness and compartment filling degree before the carrier compartments were fully willed with biomass. However, 3D imaging with OCT was capable of visualizing and quantifying the heterogeneous structure of biofilms, which cannot be achieved using 2D imaging.
KW - Biofilm structure
KW - Light microscopy
KW - Moving bed biofilm reactor
KW - Optical coherence tomography
KW - Structural parameter
UR - http://www.scopus.com/inward/record.url?scp=84944472726&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2015.10.013
DO - 10.1016/j.biortech.2015.10.013
M3 - Article
AN - SCOPUS:84944472726
SN - 0960-8524
VL - 200
SP - 128
EP - 136
JO - Bioresource Technology
JF - Bioresource Technology
ER -