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Lipid bilayers cushioned with polyelectrolyte-based films on doped silicon surfaces. / Poltorak, Lukasz; Verheijden, Mark L.; Bosma, Duco; Jonkheijm, Pascal; de Smet, Louis C.P.M.; Sudhölter, Ernst J.R.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1860, No. 12, 2018, p. 2669-2680.

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Poltorak, Lukasz ; Verheijden, Mark L. ; Bosma, Duco ; Jonkheijm, Pascal ; de Smet, Louis C.P.M. ; Sudhölter, Ernst J.R. / Lipid bilayers cushioned with polyelectrolyte-based films on doped silicon surfaces. In: Biochimica et Biophysica Acta - Biomembranes. 2018 ; Vol. 1860, No. 12. pp. 2669-2680.

BibTeX

@article{63db240999d04471b9682305485dadb5,
title = "Lipid bilayers cushioned with polyelectrolyte-based films on doped silicon surfaces",
abstract = "Silicon semiconductors with a thin surface layer of silica were first modified with polyelectrolytes (polyethyleneimine, polystyrene sulfonate and poly(allylamine)) via a facile layer-by-layer deposition approach. Subsequently, lipid vesicles were added to the preformed polymeric cushion, resulting in the adsorption of intact vesicles or fusion and lipid bilayer formation. To study involved interactions we employed optical reflectometry, electrochemical impedance spectroscopy and fluorescent recovery after photobleaching. Three phospholipids with different charge of polar head groups, i.e. 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used to prepare vesicles with varying surface charge. We observed that only lipid vesicles composed from 1:1 (mole:mole) mixture of DOPC/DOPS have the ability to fuse onto an oppositely charged terminal layer of polyelectrolyte giving a lipid bilayer with a resistance of >100 kΩ. With optical reflectometry we found that the vesicle surface charge is directly related to the amount of mass adsorbed onto the surface. An interesting observation was that zwitterionic polar head groups of DOPC allow the adsorption on both positively and negatively charged surfaces. As found with fluorescent recovery after photobleaching, positively charged surface governed by the presence of poly(allylamine) as the terminal layer resulted in intact DOPC lipid vesicles adsorption whereas in the case of a negatively charged silica surface formation of lipid bilayers was observed, as expected from literature.",
keywords = "Electrochemical impedance spectroscopy, Layer-by-layer deposition, Silicon semiconductor, Supported lipid bilayer, Thin polymeric cushion",
author = "Lukasz Poltorak and Verheijden, {Mark L.} and Duco Bosma and Pascal Jonkheijm and {de Smet}, {Louis C.P.M.} and Sudh{\"o}lter, {Ernst J.R.}",
note = "Accepted Author Manuscript",
year = "2018",
doi = "10.1016/j.bbamem.2018.09.018",
language = "English",
volume = "1860",
pages = "2669--2680",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "12",

}

RIS

TY - JOUR

T1 - Lipid bilayers cushioned with polyelectrolyte-based films on doped silicon surfaces

AU - Poltorak, Lukasz

AU - Verheijden, Mark L.

AU - Bosma, Duco

AU - Jonkheijm, Pascal

AU - de Smet, Louis C.P.M.

AU - Sudhölter, Ernst J.R.

N1 - Accepted Author Manuscript

PY - 2018

Y1 - 2018

N2 - Silicon semiconductors with a thin surface layer of silica were first modified with polyelectrolytes (polyethyleneimine, polystyrene sulfonate and poly(allylamine)) via a facile layer-by-layer deposition approach. Subsequently, lipid vesicles were added to the preformed polymeric cushion, resulting in the adsorption of intact vesicles or fusion and lipid bilayer formation. To study involved interactions we employed optical reflectometry, electrochemical impedance spectroscopy and fluorescent recovery after photobleaching. Three phospholipids with different charge of polar head groups, i.e. 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used to prepare vesicles with varying surface charge. We observed that only lipid vesicles composed from 1:1 (mole:mole) mixture of DOPC/DOPS have the ability to fuse onto an oppositely charged terminal layer of polyelectrolyte giving a lipid bilayer with a resistance of >100 kΩ. With optical reflectometry we found that the vesicle surface charge is directly related to the amount of mass adsorbed onto the surface. An interesting observation was that zwitterionic polar head groups of DOPC allow the adsorption on both positively and negatively charged surfaces. As found with fluorescent recovery after photobleaching, positively charged surface governed by the presence of poly(allylamine) as the terminal layer resulted in intact DOPC lipid vesicles adsorption whereas in the case of a negatively charged silica surface formation of lipid bilayers was observed, as expected from literature.

AB - Silicon semiconductors with a thin surface layer of silica were first modified with polyelectrolytes (polyethyleneimine, polystyrene sulfonate and poly(allylamine)) via a facile layer-by-layer deposition approach. Subsequently, lipid vesicles were added to the preformed polymeric cushion, resulting in the adsorption of intact vesicles or fusion and lipid bilayer formation. To study involved interactions we employed optical reflectometry, electrochemical impedance spectroscopy and fluorescent recovery after photobleaching. Three phospholipids with different charge of polar head groups, i.e. 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were used to prepare vesicles with varying surface charge. We observed that only lipid vesicles composed from 1:1 (mole:mole) mixture of DOPC/DOPS have the ability to fuse onto an oppositely charged terminal layer of polyelectrolyte giving a lipid bilayer with a resistance of >100 kΩ. With optical reflectometry we found that the vesicle surface charge is directly related to the amount of mass adsorbed onto the surface. An interesting observation was that zwitterionic polar head groups of DOPC allow the adsorption on both positively and negatively charged surfaces. As found with fluorescent recovery after photobleaching, positively charged surface governed by the presence of poly(allylamine) as the terminal layer resulted in intact DOPC lipid vesicles adsorption whereas in the case of a negatively charged silica surface formation of lipid bilayers was observed, as expected from literature.

KW - Electrochemical impedance spectroscopy

KW - Layer-by-layer deposition

KW - Silicon semiconductor

KW - Supported lipid bilayer

KW - Thin polymeric cushion

UR - http://www.scopus.com/inward/record.url?scp=85054809052&partnerID=8YFLogxK

U2 - 10.1016/j.bbamem.2018.09.018

DO - 10.1016/j.bbamem.2018.09.018

M3 - Article

VL - 1860

SP - 2669

EP - 2680

JO - Biochimica et Biophysica Acta - Biomembranes

T2 - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 12

ER -

ID: 47168935