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Memory CD4+ T cells are generated in the human fetal intestine. / Li, Na; van Unen, Vincent; Abdelaal, Tamim; Guo, Nannan; Kasatskaya, Sofya A.; Chuva de Sousa Lopes, Susana M.; Höllt, Thomas; Britanova, Olga V.; Lelieveldt, Boudewijn P.F.; More Authors.

In: Nature Immunology, Vol. 20, No. 3, 2019, p. 301-312.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Li, N, van Unen, V, Abdelaal, T, Guo, N, Kasatskaya, SA, Chuva de Sousa Lopes, SM, Höllt, T, Britanova, OV, Lelieveldt, BPF & More Authors 2019, 'Memory CD4+ T cells are generated in the human fetal intestine' Nature Immunology, vol. 20, no. 3, pp. 301-312. https://doi.org/10.1038/s41590-018-0294-9

APA

Li, N., van Unen, V., Abdelaal, T., Guo, N., Kasatskaya, S. A., Chuva de Sousa Lopes, S. M., ... More Authors (2019). Memory CD4+ T cells are generated in the human fetal intestine. Nature Immunology, 20(3), 301-312. https://doi.org/10.1038/s41590-018-0294-9

Vancouver

Author

Li, Na ; van Unen, Vincent ; Abdelaal, Tamim ; Guo, Nannan ; Kasatskaya, Sofya A. ; Chuva de Sousa Lopes, Susana M. ; Höllt, Thomas ; Britanova, Olga V. ; Lelieveldt, Boudewijn P.F. ; More Authors. / Memory CD4+ T cells are generated in the human fetal intestine. In: Nature Immunology. 2019 ; Vol. 20, No. 3. pp. 301-312.

BibTeX

@article{94c5eb68842a4f46ac9bdc747566b83b,
title = "Memory CD4+ T cells are generated in the human fetal intestine",
abstract = "The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO+ T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4+ T cell compartment in the human fetal intestine. We identified 22 CD4+ T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4+ T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-γ and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4+ T cells. Imaging mass cytometry indicated that memory-like CD4+ T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens.",
author = "Na Li and {van Unen}, Vincent and Tamim Abdelaal and Nannan Guo and Kasatskaya, {Sofya A.} and {Chuva de Sousa Lopes}, {Susana M.} and Thomas H{\"o}llt and Britanova, {Olga V.} and Lelieveldt, {Boudewijn P.F.} and {More Authors}",
year = "2019",
doi = "10.1038/s41590-018-0294-9",
language = "English",
volume = "20",
pages = "301--312",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "3",

}

RIS

TY - JOUR

T1 - Memory CD4+ T cells are generated in the human fetal intestine

AU - Li, Na

AU - van Unen, Vincent

AU - Abdelaal, Tamim

AU - Guo, Nannan

AU - Kasatskaya, Sofya A.

AU - Chuva de Sousa Lopes, Susana M.

AU - Höllt, Thomas

AU - Britanova, Olga V.

AU - Lelieveldt, Boudewijn P.F.

AU - More Authors, null

PY - 2019

Y1 - 2019

N2 - The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO+ T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4+ T cell compartment in the human fetal intestine. We identified 22 CD4+ T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4+ T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-γ and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4+ T cells. Imaging mass cytometry indicated that memory-like CD4+ T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

AB - The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO+ T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4+ T cell compartment in the human fetal intestine. We identified 22 CD4+ T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4+ T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-γ and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4+ T cells. Imaging mass cytometry indicated that memory-like CD4+ T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4+ T cells in the human fetal intestine that is consistent with exposure to foreign antigens.

UR - http://www.scopus.com/inward/record.url?scp=85060343432&partnerID=8YFLogxK

U2 - 10.1038/s41590-018-0294-9

DO - 10.1038/s41590-018-0294-9

M3 - Article

VL - 20

SP - 301

EP - 312

JO - Nature Immunology

T2 - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 3

ER -

ID: 51367606