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Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. / Quirós-Solano, W. F.; Gaio, N.; Stassen, O.M.J.A.; Arik, Y.B.; Silvestri, C.; Van Engeland, N.C.A.; Van der Meer, A.; Passier, R.; Sahlgren, C.M.; Bouten, C.V.C.; van den Berg, A.; Dekker, R.; Sarro, P.M.

In: Scientific Reports, Vol. 8, 13524, 2018, p. 1-11.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Quirós-Solano, WF, Gaio, N, Stassen, OMJA, Arik, YB, Silvestri, C, Van Engeland, NCA, Van der Meer, A, Passier, R, Sahlgren, CM, Bouten, CVC, van den Berg, A, Dekker, R & Sarro, PM 2018, 'Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips' Scientific Reports, vol. 8, 13524, pp. 1-11. https://doi.org/10.1038/s41598-018-31912-6

APA

Vancouver

Author

Quirós-Solano, W. F. ; Gaio, N. ; Stassen, O.M.J.A. ; Arik, Y.B. ; Silvestri, C. ; Van Engeland, N.C.A. ; Van der Meer, A. ; Passier, R. ; Sahlgren, C.M. ; Bouten, C.V.C. ; van den Berg, A. ; Dekker, R. ; Sarro, P.M. / Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips. In: Scientific Reports. 2018 ; Vol. 8. pp. 1-11.

BibTeX

@article{f9f4385eb8734c169419f7bd466d3e02,
title = "Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips",
abstract = "We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65{\%}) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85{\%}). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.",
keywords = "OA-Fund TU Delft",
author = "Quir{\'o}s-Solano, {W. F.} and N. Gaio and O.M.J.A. Stassen and Y.B. Arik and C. Silvestri and {Van Engeland}, N.C.A. and {Van der Meer}, A. and R. Passier and C.M. Sahlgren and C.V.C. Bouten and {van den Berg}, A. and R. Dekker and P.M. Sarro",
year = "2018",
doi = "10.1038/s41598-018-31912-6",
language = "English",
volume = "8",
pages = "1--11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Microfabricated tuneable and transferable porous PDMS membranes for Organs-on-Chips

AU - Quirós-Solano, W. F.

AU - Gaio, N.

AU - Stassen, O.M.J.A.

AU - Arik, Y.B.

AU - Silvestri, C.

AU - Van Engeland, N.C.A.

AU - Van der Meer, A.

AU - Passier, R.

AU - Sahlgren, C.M.

AU - Bouten, C.V.C.

AU - van den Berg, A.

AU - Dekker, R.

AU - Sarro, P.M.

PY - 2018

Y1 - 2018

N2 - We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65%) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85%). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.

AB - We present a novel and highly reproducible process to fabricate transferable porous PDMS membranes for PDMS-based Organs-on-Chips (OOCs) using microelectromechanical systems (MEMS) fabrication technologies. Porous PDMS membranes with pore sizes down to 2.0 μm in diameter and a wide porosity range (2–65%) can be fabricated. To overcome issues normally faced when using replica moulding and extend the applicability to most OOCs and improve their scalability and reproducibility, the process includes a sacrificial layer to easily transfer the membranes from a silicon carrier to any PDMS-based OOC. The highly reliable fabrication and transfer method does not need of manual handling to define the pore features (size, distribution), allowing very thin (<10 μm) functional membranes to be transferred at chip level with a high success rate (85%). The viability of cell culturing on the porous membranes was assessed by culturing two different cell types on transferred membranes in two different OOCs. Human umbilical endothelial cells (HUVEC) and MDA-MB-231 (MDA) cells were successfully cultured confirming the viability of cell culturing and the biocompatibility of the membranes. The results demonstrate the potential of controlling the porous membrane features to study cell mechanisms such as transmigrations, monolayer formation, and barrier function. The high control over the membrane characteristics might consequently allow to intentionally trigger or prevent certain cellular responses or mechanisms when studying human physiology and pathology using OOCs.

KW - OA-Fund TU Delft

UR - http://www.scopus.com/inward/record.url?scp=85053186916&partnerID=8YFLogxK

UR - http://resolver.tudelft.nl/uuid:f9f4385e-b873-4c16-9419-f7bd466d3e02

U2 - 10.1038/s41598-018-31912-6

DO - 10.1038/s41598-018-31912-6

M3 - Article

VL - 8

SP - 1

EP - 11

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 13524

ER -

ID: 46838032