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Modeling cell-substrate de-adhesion dynamics under fluid shear. / Maan, Renu; Rani, Garima; Menon, Gautam I.; Pullarkat, Pramod A.

In: Physical Biology, Vol. 15, No. 4, 046006, 2018.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Maan, R, Rani, G, Menon, GI & Pullarkat, PA 2018, 'Modeling cell-substrate de-adhesion dynamics under fluid shear' Physical Biology, vol. 15, no. 4, 046006. https://doi.org/10.1088/1478-3975/aabc66

APA

Maan, R., Rani, G., Menon, G. I., & Pullarkat, P. A. (2018). Modeling cell-substrate de-adhesion dynamics under fluid shear. Physical Biology, 15(4), [046006]. https://doi.org/10.1088/1478-3975/aabc66

Vancouver

Maan R, Rani G, Menon GI, Pullarkat PA. Modeling cell-substrate de-adhesion dynamics under fluid shear. Physical Biology. 2018;15(4). 046006. https://doi.org/10.1088/1478-3975/aabc66

Author

Maan, Renu ; Rani, Garima ; Menon, Gautam I. ; Pullarkat, Pramod A. / Modeling cell-substrate de-adhesion dynamics under fluid shear. In: Physical Biology. 2018 ; Vol. 15, No. 4.

BibTeX

@article{2ba4b5b3d10148f5a29f994250b54127,
title = "Modeling cell-substrate de-adhesion dynamics under fluid shear",
abstract = "Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of ∼500 pN associated to a single focal contact, and characteristic timescales of 190 ≤τ≤ 350 s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.",
keywords = "adhesion strength, cell de-adhesion, cell detachment model, cell shearing, mechanobiology",
author = "Renu Maan and Garima Rani and Menon, {Gautam I.} and Pullarkat, {Pramod A.}",
year = "2018",
doi = "10.1088/1478-3975/aabc66",
language = "English",
volume = "15",
journal = "Physical Biology",
issn = "1478-3967",
publisher = "IOP Publishing Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Modeling cell-substrate de-adhesion dynamics under fluid shear

AU - Maan, Renu

AU - Rani, Garima

AU - Menon, Gautam I.

AU - Pullarkat, Pramod A.

PY - 2018

Y1 - 2018

N2 - Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of ∼500 pN associated to a single focal contact, and characteristic timescales of 190 ≤τ≤ 350 s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.

AB - Changes in cell-substrate adhesion are believed to signal the onset of cancer metastasis, but such changes must be quantified against background levels of intrinsic heterogeneity between cells. Variations in cell-substrate adhesion strengths can be probed through biophysical measurements of cell detachment from substrates upon the application of an external force. Here, we investigate, theoretically and experimentally, the detachment of cells adhered to substrates when these cells are subjected to fluid shear. We present a theoretical framework within which we calculate the fraction of detached cells as a function of shear stress for fast ramps as well as the decay in this fraction at fixed shear stress as a function of time. Using HEK and 3T3 fibroblast cells as experimental model systems, we extract characteristic force scales for cell adhesion as well as characteristic detachment times. We estimate force-scales of ∼500 pN associated to a single focal contact, and characteristic timescales of 190 ≤τ≤ 350 s representing cell-spread-area dependent mean first passage times to the detached state at intermediate values of the shear stress. Variations in adhesion across cell types are especially prominent when cell detachment is probed by applying a time-varying shear stress. These methods can be applied to characterizing changes in cell adhesion in a variety of contexts, including metastasis.

KW - adhesion strength

KW - cell de-adhesion

KW - cell detachment model

KW - cell shearing

KW - mechanobiology

UR - http://www.scopus.com/inward/record.url?scp=85047445323&partnerID=8YFLogxK

U2 - 10.1088/1478-3975/aabc66

DO - 10.1088/1478-3975/aabc66

M3 - Article

VL - 15

JO - Physical Biology

T2 - Physical Biology

JF - Physical Biology

SN - 1478-3967

IS - 4

M1 - 046006

ER -

ID: 45256490