TY - JOUR
T1 - Multiscale 3D-printing of microfluidic AFM cantilevers
AU - Kramer, Robert C.L.N.
AU - Verlinden, Eleonoor J.
AU - Angeloni, Livia
AU - Van Den Heuvel, Anita
AU - Fratila-Apachitei, Lidy E.
AU - Van Der Maarel, Silvère M.
AU - Ghatkesar, Murali K.
PY - 2020
Y1 - 2020
N2 - Microfluidic atomic force microscopy (AFM) cantilever probes have all the functionalities of a standard AFM cantilever along with fluid pipetting. They have a channel inside the cantilever and an aperture at the tip. Such probes are useful for precise fluid manipulation at a desired location, for example near or inside cells. They are typically made by complex microfabrication process steps, resulting in expensive probes. Here, we used two different 3D additive manufacturing techniques, stereolithography and two-photon polymerization, to directly print ready-to-use microfluidic AFM cantilever probes. This approach has considerably reduced the fabrication time and increased the design freedom. One of the probes, 564 μm long, 30 μm wide, 30 μm high, with a 25 μm diameter channel and 2.5 μm wall thickness had a spring constant of 3.7 N m-1 and the polymer fabrication material had an elastic modulus of 4.2 GPa. Using these 3D printed probes, AFM imaging of a surface, puncturing of the cell membrane, and aspiration at the single cell level have been demonstrated.
AB - Microfluidic atomic force microscopy (AFM) cantilever probes have all the functionalities of a standard AFM cantilever along with fluid pipetting. They have a channel inside the cantilever and an aperture at the tip. Such probes are useful for precise fluid manipulation at a desired location, for example near or inside cells. They are typically made by complex microfabrication process steps, resulting in expensive probes. Here, we used two different 3D additive manufacturing techniques, stereolithography and two-photon polymerization, to directly print ready-to-use microfluidic AFM cantilever probes. This approach has considerably reduced the fabrication time and increased the design freedom. One of the probes, 564 μm long, 30 μm wide, 30 μm high, with a 25 μm diameter channel and 2.5 μm wall thickness had a spring constant of 3.7 N m-1 and the polymer fabrication material had an elastic modulus of 4.2 GPa. Using these 3D printed probes, AFM imaging of a surface, puncturing of the cell membrane, and aspiration at the single cell level have been demonstrated.
UR - http://www.scopus.com/inward/record.url?scp=85078211847&partnerID=8YFLogxK
U2 - 10.1039/c9lc00668k
DO - 10.1039/c9lc00668k
M3 - Article
C2 - 31808485
SN - 1473-0197
VL - 20
SP - 311
EP - 319
JO - Lab on a Chip
JF - Lab on a Chip
IS - 2
ER -