Abstract
We present a novel microfluidic system for the pH-gradient focusing of proteins with the integration of 16 parallel micro-mixers, a micro-column, and a multiplexer. In this work we successfully achieved the creation of 16 non-linear gradients and the generation of a solid-phase micro-column for the realization of anion exchange chromatography on a single chip. With the device we demonstrated the separation of a protein mixture of R-phycoerythrin and FITC-BSA based on pH-gradient chromatofocusing.
Original language | English |
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Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | Chemical and Biological Microsystems Society |
Pages | 245-247 |
Number of pages | 3 |
ISBN (Electronic) | 9780979806483 |
Publication status | Published - 2015 |
Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Conference
Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
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Country/Territory | Korea, Republic of |
City | Gyeongju |
Period | 25/10/15 → 29/10/15 |
Keywords
- Chromatofocusing
- Micro-column
- Microfluidics
- PH gradient