Preparation of D-xylulose from D-xylose

Jack Pronk, A.W. Bakker, H.E. van Dam, A.J.J. Straathof, W.A. Scheffers, J.P. van Dijken

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

A simple method is described for the preparation of d-xylulose. It consists of the isomerization of d-xylose with xylose isomerase (EC 5.3.1.5), yielding an equilibrium mixture of d-xylulose and d-xylose. This is followed by the quantitative oxidation of residual d-xylose to d-xylonic acid with immobilized A. calcoaceticus cells. A combination of methanol precipitation and ion exchange is used for the removal of xylonic acid. This procedure offers many advantages over existing methods for the preparation of d-xylulose. The purity of the final product compares favorably to that of a commercial d-xylulose preparation.
Original languageEnglish
Pages (from-to)537-542
JournalEnzyme and Microbial Technology: biotechnology research and reviews
Volume10
Issue number9
DOIs
Publication statusPublished - 1988

Keywords

  • d-xylulose
  • xylose isomerase
  • Acinetobacter calcoaceticus
  • immobilization

Fingerprint

Dive into the research topics of 'Preparation of D-xylulose from D-xylose'. Together they form a unique fingerprint.

Cite this