Pupil mask diversity for image correction in microscopy

Dean Wilding; Paolo Pozzi; Oleg Soloviev; Gleb Vdovin; Michel Verhaegen

doi:10.1364/OE.26.014832

Pupil mask diversity for image correction in microscopy

Dean Wilding^*, Paolo Pozzi, Oleg Soloviev, Gleb Vdovin, Michel Verhaegen

^*Corresponding author for this work

Team Raf Van de Plas

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned in several di erent orientations within the pupil, followed by computer processing of the diversity images. The method has proved to be fast, easy to implement, and cost-e ective in high-resolution imaging of casper fli:GFP zebrafish.

Original language	English
Pages (from-to)	14832-14841
Journal	Optics Express
Volume	26
Issue number	12
DOIs	https://doi.org/10.1364/OE.26.014832
Publication status	Published - 2018

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oe-26-12-14832Licence: CC BY

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AU - Pozzi, Paolo

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AU - Vdovin, Gleb

AU - Verhaegen, Michel

PY - 2018

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Pupil mask diversity for image correction in microscopy

Abstract

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