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Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance. / van Oene, Maarten M.; Ha, Seungkyu; Jager, Tessa; Lee, Mina; Pedaci, Francesco; Lipfert, Jan; Dekker, Nynke H.

In: Biophysical Journal, Vol. 114, No. 8, 24.04.2018, p. 1970-1979.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

van Oene, MM, Ha, S, Jager, T, Lee, M, Pedaci, F, Lipfert, J & Dekker, NH 2018, 'Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance', Biophysical Journal, vol. 114, no. 8, pp. 1970-1979. https://doi.org/10.1016/j.bpj.2018.02.039

APA

van Oene, M. M., Ha, S., Jager, T., Lee, M., Pedaci, F., Lipfert, J., & Dekker, N. H. (2018). Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance. Biophysical Journal, 114(8), 1970-1979. https://doi.org/10.1016/j.bpj.2018.02.039

Vancouver

van Oene MM, Ha S, Jager T, Lee M, Pedaci F, Lipfert J et al. Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance. Biophysical Journal. 2018 Apr 24;114(8):1970-1979. https://doi.org/10.1016/j.bpj.2018.02.039

Author

van Oene, Maarten M. ; Ha, Seungkyu ; Jager, Tessa ; Lee, Mina ; Pedaci, Francesco ; Lipfert, Jan ; Dekker, Nynke H. / Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance. In: Biophysical Journal. 2018 ; Vol. 114, No. 8. pp. 1970-1979.

BibTeX

@article{c1ee4c4865f04848bfbb0a811c69057a,
title = "Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance",
abstract = "Single-molecule manipulation techniques have provided unprecedented insights into the structure, function, interactions, and mechanical properties of biological macromolecules. Recently, the single-molecule toolbox has been expanded by techniques that enable measurements of rotation and torque, such as the optical torque wrench (OTW) and several different implementations of magnetic (torque) tweezers. Although systematic analyses of the position and force precision of single-molecule techniques have attracted considerable attention, their angle and torque precision have been treated in much less detail. Here, we propose Allan deviation as a tool to systematically quantitate angle and torque precision in single-molecule measurements. We apply the Allan variance method to experimental data from our implementations of (electro)magnetic torque tweezers and an OTW and find that both approaches can achieve a torque precision better than 1 pN · nm. The OTW, capable of measuring torque on (sub)millisecond timescales, provides the best torque precision for measurement times ≲10 s, after which drift becomes a limiting factor. For longer measurement times, magnetic torque tweezers with their superior stability provide the best torque precision. Use of the Allan deviation enables critical assessments of the torque precision as a function of measurement time across different measurement modalities and provides a tool to optimize measurement protocols for a given instrument and application.",
author = "{van Oene}, {Maarten M.} and Seungkyu Ha and Tessa Jager and Mina Lee and Francesco Pedaci and Jan Lipfert and Dekker, {Nynke H.}",
note = "Accepted Author Manuscript",
year = "2018",
month = apr,
day = "24",
doi = "10.1016/j.bpj.2018.02.039",
language = "English",
volume = "114",
pages = "1970--1979",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Biophysical Society",
number = "8",

}

RIS

TY - JOUR

T1 - Quantifying the Precision of Single-Molecule Torque and Twist Measurements Using Allan Variance

AU - van Oene, Maarten M.

AU - Ha, Seungkyu

AU - Jager, Tessa

AU - Lee, Mina

AU - Pedaci, Francesco

AU - Lipfert, Jan

AU - Dekker, Nynke H.

N1 - Accepted Author Manuscript

PY - 2018/4/24

Y1 - 2018/4/24

N2 - Single-molecule manipulation techniques have provided unprecedented insights into the structure, function, interactions, and mechanical properties of biological macromolecules. Recently, the single-molecule toolbox has been expanded by techniques that enable measurements of rotation and torque, such as the optical torque wrench (OTW) and several different implementations of magnetic (torque) tweezers. Although systematic analyses of the position and force precision of single-molecule techniques have attracted considerable attention, their angle and torque precision have been treated in much less detail. Here, we propose Allan deviation as a tool to systematically quantitate angle and torque precision in single-molecule measurements. We apply the Allan variance method to experimental data from our implementations of (electro)magnetic torque tweezers and an OTW and find that both approaches can achieve a torque precision better than 1 pN · nm. The OTW, capable of measuring torque on (sub)millisecond timescales, provides the best torque precision for measurement times ≲10 s, after which drift becomes a limiting factor. For longer measurement times, magnetic torque tweezers with their superior stability provide the best torque precision. Use of the Allan deviation enables critical assessments of the torque precision as a function of measurement time across different measurement modalities and provides a tool to optimize measurement protocols for a given instrument and application.

AB - Single-molecule manipulation techniques have provided unprecedented insights into the structure, function, interactions, and mechanical properties of biological macromolecules. Recently, the single-molecule toolbox has been expanded by techniques that enable measurements of rotation and torque, such as the optical torque wrench (OTW) and several different implementations of magnetic (torque) tweezers. Although systematic analyses of the position and force precision of single-molecule techniques have attracted considerable attention, their angle and torque precision have been treated in much less detail. Here, we propose Allan deviation as a tool to systematically quantitate angle and torque precision in single-molecule measurements. We apply the Allan variance method to experimental data from our implementations of (electro)magnetic torque tweezers and an OTW and find that both approaches can achieve a torque precision better than 1 pN · nm. The OTW, capable of measuring torque on (sub)millisecond timescales, provides the best torque precision for measurement times ≲10 s, after which drift becomes a limiting factor. For longer measurement times, magnetic torque tweezers with their superior stability provide the best torque precision. Use of the Allan deviation enables critical assessments of the torque precision as a function of measurement time across different measurement modalities and provides a tool to optimize measurement protocols for a given instrument and application.

UR - http://resolver.tudelft.nl/uuid:c1ee4c48-65f0-4848-bfbb-0a811c69057a

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U2 - 10.1016/j.bpj.2018.02.039

DO - 10.1016/j.bpj.2018.02.039

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AN - SCOPUS:85046779163

VL - 114

SP - 1970

EP - 1979

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 8

ER -

ID: 45139117