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Shape and Size Control of Artificial Cells for Bottom-Up Biology. / Fanalista, Federico; Birnie, Anthony; Maan, Renu; Burla, Federica; Charles, Kevin; Pawlik, Grzegorz; Deshpande, Siddharth; Koenderink, Gijsje H.; Dogterom, Marileen; Dekker, Cees.

In: ACS Nano, Vol. 13, No. 5, 2019, p. 5439-5450.

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Fanalista, Federico ; Birnie, Anthony ; Maan, Renu ; Burla, Federica ; Charles, Kevin ; Pawlik, Grzegorz ; Deshpande, Siddharth ; Koenderink, Gijsje H. ; Dogterom, Marileen ; Dekker, Cees. / Shape and Size Control of Artificial Cells for Bottom-Up Biology. In: ACS Nano. 2019 ; Vol. 13, No. 5. pp. 5439-5450.

BibTeX

@article{f1d33cea20854f689b8cae6044a5caa5,
title = "Shape and Size Control of Artificial Cells for Bottom-Up Biology",
abstract = "Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components in synthetic cells. As encapsulation and controlled manipulation of these elements is a crucial step in the recreation of such cell-like objects, microfluidics is increasingly used for the production of minimal artificial containers such as single-emulsion droplets, double-emulsion droplets, and liposomes. Despite the importance of cell morphology on cellular dynamics, current synthetic-cell studies mainly use spherical containers, and methods to actively shape manipulate these have been lacking. In this paper, we describe a microfluidic platform to deform the shape of artificial cells into a variety of shapes (rods and discs) with adjustable cell-like dimensions below 5 μm, thereby mimicking realistic cell morphologies. To illustrate the potential of our method, we reconstitute three biologically relevant protein systems (FtsZ, microtubules, collagen) inside rod-shaped containers and study the arrangement of the protein networks inside these synthetic containers with physiologically relevant morphologies resembling those found in living cells.",
keywords = "bottom-up biology, droplets, lab-on-a-chip, liposomes, microfluidics, morphology control, synthetic cell",
author = "Federico Fanalista and Anthony Birnie and Renu Maan and Federica Burla and Kevin Charles and Grzegorz Pawlik and Siddharth Deshpande and Koenderink, {Gijsje H.} and Marileen Dogterom and Cees Dekker",
year = "2019",
doi = "10.1021/acsnano.9b00220",
language = "English",
volume = "13",
pages = "5439--5450",
journal = "ACS Nano (online)",
issn = "1936-086X",
publisher = "American Chemical Society (ACS)",
number = "5",

}

RIS

TY - JOUR

T1 - Shape and Size Control of Artificial Cells for Bottom-Up Biology

AU - Fanalista, Federico

AU - Birnie, Anthony

AU - Maan, Renu

AU - Burla, Federica

AU - Charles, Kevin

AU - Pawlik, Grzegorz

AU - Deshpande, Siddharth

AU - Koenderink, Gijsje H.

AU - Dogterom, Marileen

AU - Dekker, Cees

PY - 2019

Y1 - 2019

N2 - Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components in synthetic cells. As encapsulation and controlled manipulation of these elements is a crucial step in the recreation of such cell-like objects, microfluidics is increasingly used for the production of minimal artificial containers such as single-emulsion droplets, double-emulsion droplets, and liposomes. Despite the importance of cell morphology on cellular dynamics, current synthetic-cell studies mainly use spherical containers, and methods to actively shape manipulate these have been lacking. In this paper, we describe a microfluidic platform to deform the shape of artificial cells into a variety of shapes (rods and discs) with adjustable cell-like dimensions below 5 μm, thereby mimicking realistic cell morphologies. To illustrate the potential of our method, we reconstitute three biologically relevant protein systems (FtsZ, microtubules, collagen) inside rod-shaped containers and study the arrangement of the protein networks inside these synthetic containers with physiologically relevant morphologies resembling those found in living cells.

AB - Bottom-up biology is an expanding research field that aims to understand the mechanisms underlying biological processes via in vitro assembly of their essential components in synthetic cells. As encapsulation and controlled manipulation of these elements is a crucial step in the recreation of such cell-like objects, microfluidics is increasingly used for the production of minimal artificial containers such as single-emulsion droplets, double-emulsion droplets, and liposomes. Despite the importance of cell morphology on cellular dynamics, current synthetic-cell studies mainly use spherical containers, and methods to actively shape manipulate these have been lacking. In this paper, we describe a microfluidic platform to deform the shape of artificial cells into a variety of shapes (rods and discs) with adjustable cell-like dimensions below 5 μm, thereby mimicking realistic cell morphologies. To illustrate the potential of our method, we reconstitute three biologically relevant protein systems (FtsZ, microtubules, collagen) inside rod-shaped containers and study the arrangement of the protein networks inside these synthetic containers with physiologically relevant morphologies resembling those found in living cells.

KW - bottom-up biology

KW - droplets

KW - lab-on-a-chip

KW - liposomes

KW - microfluidics

KW - morphology control

KW - synthetic cell

UR - http://www.scopus.com/inward/record.url?scp=85066822478&partnerID=8YFLogxK

U2 - 10.1021/acsnano.9b00220

DO - 10.1021/acsnano.9b00220

M3 - Article

VL - 13

SP - 5439

EP - 5450

JO - ACS Nano (online)

T2 - ACS Nano (online)

JF - ACS Nano (online)

SN - 1936-086X

IS - 5

ER -

ID: 55117147