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Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge. / de Graaff, Danny R.; Felz, Simon; Neu, Thomas R.; Pronk, Mario; van Loosdrecht, Mark C.M.; Lin, Yuemei.

In: Water Research, Vol. 155, 2019, p. 343-351.

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@article{d19bd6f5dc1348ccb625c20846fcb74f,
title = "Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge",
abstract = "Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases. In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done. Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter. FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars. Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains. This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.",
keywords = "Aerobic granular sludge, EPS, Glycoproteins, Neuraminic acid, Salinity, Seawater, Sialic acids",
author = "{de Graaff}, {Danny R.} and Simon Felz and Neu, {Thomas R.} and Mario Pronk and {van Loosdrecht}, {Mark C.M.} and Yuemei Lin",
year = "2019",
doi = "10.1016/j.watres.2019.02.040",
language = "English",
volume = "155",
pages = "343--351",
journal = "Water Research",
issn = "0043-1354",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge

AU - de Graaff, Danny R.

AU - Felz, Simon

AU - Neu, Thomas R.

AU - Pronk, Mario

AU - van Loosdrecht, Mark C.M.

AU - Lin, Yuemei

PY - 2019

Y1 - 2019

N2 - Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases. In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done. Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter. FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars. Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains. This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.

AB - Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases. In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done. Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter. FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars. Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains. This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge.

KW - Aerobic granular sludge

KW - EPS

KW - Glycoproteins

KW - Neuraminic acid

KW - Salinity

KW - Seawater

KW - Sialic acids

UR - http://www.scopus.com/inward/record.url?scp=85062476597&partnerID=8YFLogxK

U2 - 10.1016/j.watres.2019.02.040

DO - 10.1016/j.watres.2019.02.040

M3 - Article

VL - 155

SP - 343

EP - 351

JO - Water Research

T2 - Water Research

JF - Water Research

SN - 0043-1354

ER -

ID: 52089117