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Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels. / Marin, Victor; Kieffer, Roland; Padmos, Raymond; Aubin-Tam, Marie Eve.

In: Analytical Chemistry, Vol. 88, No. 15, 28.06.2016, p. 7466-7470.

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Marin, Victor ; Kieffer, Roland ; Padmos, Raymond ; Aubin-Tam, Marie Eve. / Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels. In: Analytical Chemistry. 2016 ; Vol. 88, No. 15. pp. 7466-7470.

BibTeX

@article{d32e20589ef74b4c949d4fe6a2a3c662,
title = "Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels",
abstract = "We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.",
author = "Victor Marin and Roland Kieffer and Raymond Padmos and Aubin-Tam, {Marie Eve}",
note = "Accepted Author Manuscript",
year = "2016",
month = "6",
day = "28",
doi = "10.1021/acs.analchem.6b00926",
language = "English",
volume = "88",
pages = "7466--7470",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "15",

}

RIS

TY - JOUR

T1 - Stable Free-Standing Lipid Bilayer Membranes in Norland Optical Adhesive 81 Microchannels

AU - Marin, Victor

AU - Kieffer, Roland

AU - Padmos, Raymond

AU - Aubin-Tam, Marie Eve

N1 - Accepted Author Manuscript

PY - 2016/6/28

Y1 - 2016/6/28

N2 - We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.

AB - We report a simple, cost-effective, and reproducible method to form free-standing lipid bilayer membranes in microdevices made with Norland Optical Adhesive 81 (NOA81). Surface treatment with either alkylsilane or fluoroalkylsilane enables the self-assembly of stable 1,2-diphytanoyl-sn-glycero-3-phosphocholine 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dihexadecanoyl-sn-glycero-3-phosphocholine (DPPC) membranes. Capacitance measurements are used to characterize the lipid bilayer and to follow its formation in real-time. With current recordings, we detect the insertion of single α-hemolysin pores into the bilayer membrane, demonstrating the possibility of using this device for single-channel electrophysiology sensing applications. Optical transparency of the device and vertical position of the lipid bilayer with respect to the microscope focal plane allows easy integration with other single-molecule techniques, such as optical tweezers. Therefore, this method to form long-lived lipid bilayers finds a wide range of applications, from sensing measurements to biophysical studies of lipid bilayers and associated proteins.

UR - http://www.scopus.com/inward/record.url?scp=84980416309&partnerID=8YFLogxK

UR - http://resolver.tudelft.nl/uuid:d32e2058-9ef7-4b4c-949d-4fe6a2a3c662

U2 - 10.1021/acs.analchem.6b00926

DO - 10.1021/acs.analchem.6b00926

M3 - Article

VL - 88

SP - 7466

EP - 7470

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 15

ER -

ID: 7336101