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Submicron patterns-on-a-chip : Fabrication of a microfluidic device incorporating 3D printed surface ornaments. / Nouri-Goushki, Mahdiyeh; Sharma, Abhishek; Sasso, Luigi; Zhang, Shuang; Van Der Eerden, Bram C.J.; Staufer, Urs; Fratila-Apachitei, Lidy E.; Zadpoor, Amir A.

In: ACS Biomaterials Science and Engineering, Vol. 5, No. 11, 2019, p. 6127-6136.

Research output: Contribution to journalArticleScientificpeer-review

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Nouri-Goushki M, Sharma A, Sasso L, Zhang S, Van Der Eerden BCJ, Staufer U et al. Submicron patterns-on-a-chip: Fabrication of a microfluidic device incorporating 3D printed surface ornaments. ACS Biomaterials Science and Engineering. 2019;5(11):6127-6136. https://doi.org/10.1021/acsbiomaterials.9b01155

Author

Nouri-Goushki, Mahdiyeh ; Sharma, Abhishek ; Sasso, Luigi ; Zhang, Shuang ; Van Der Eerden, Bram C.J. ; Staufer, Urs ; Fratila-Apachitei, Lidy E. ; Zadpoor, Amir A. / Submicron patterns-on-a-chip : Fabrication of a microfluidic device incorporating 3D printed surface ornaments. In: ACS Biomaterials Science and Engineering. 2019 ; Vol. 5, No. 11. pp. 6127-6136.

BibTeX

@article{b00dab798e1248f990471be66350cbd0,
title = "Submicron patterns-on-a-chip: Fabrication of a microfluidic device incorporating 3D printed surface ornaments",
abstract = "Manufacturing high throughput in vitro models resembling the tissue microenvironment is highly demanded for studying bone regeneration. Tissues such as bone have complex multiscale architectures inside which cells reside. To this end, engineering a microfluidic platform incorporated with three-dimensional (3D) microscaffolds and submicron/nanoscale topographies can provide a promising model for 3D cell cultures. There are, however, certain challenges associated with this goal, such as the need to decorate large surfaces area with high-fidelity 3D submicron structures. Here, we succeeded in fabricating a microfluidic platform embedded with a large area (mm range) of reproducible submicron pillar-based topographies. Using the two-photon polymerization (2PP) as a 3D printing technique based on direct laser writing, uniform submicron patterns were created through optimization of the process parameters and writing strategy. To demonstrate the multiscale fabrication capabilities of this approach, submicron pillars of various heights were integrated onto the surfaces of a 3D microscaffold in a single-step 2PP process. The created submicron topography was also found to improve the hydrophilicity of the surface while being able to withstand flow rates of up to 8 mL/min. The material (IP-Dip resin) used for patterning did not have cytotoxic effects against human mesenchymal stromal cells after 3 days of dynamic culture in the microfluidic device. This proof-of-principle study, therefore, marks a significant step forward in manufacturing submicron structure-on-a-chip models for bone regeneration studies.",
keywords = "bone regeneration, microfluidics, submicron pillars, two-photon polymerization",
author = "Mahdiyeh Nouri-Goushki and Abhishek Sharma and Luigi Sasso and Shuang Zhang and {Van Der Eerden}, {Bram C.J.} and Urs Staufer and Fratila-Apachitei, {Lidy E.} and Zadpoor, {Amir A.}",
year = "2019",
doi = "10.1021/acsbiomaterials.9b01155",
language = "English",
volume = "5",
pages = "6127--6136",
journal = "ACS Biomaterials Science and Engineering",
issn = "2373-9878",
publisher = "American Chemical Society (ACS)",
number = "11",

}

RIS

TY - JOUR

T1 - Submicron patterns-on-a-chip

T2 - ACS Biomaterials Science and Engineering

AU - Nouri-Goushki, Mahdiyeh

AU - Sharma, Abhishek

AU - Sasso, Luigi

AU - Zhang, Shuang

AU - Van Der Eerden, Bram C.J.

AU - Staufer, Urs

AU - Fratila-Apachitei, Lidy E.

AU - Zadpoor, Amir A.

PY - 2019

Y1 - 2019

N2 - Manufacturing high throughput in vitro models resembling the tissue microenvironment is highly demanded for studying bone regeneration. Tissues such as bone have complex multiscale architectures inside which cells reside. To this end, engineering a microfluidic platform incorporated with three-dimensional (3D) microscaffolds and submicron/nanoscale topographies can provide a promising model for 3D cell cultures. There are, however, certain challenges associated with this goal, such as the need to decorate large surfaces area with high-fidelity 3D submicron structures. Here, we succeeded in fabricating a microfluidic platform embedded with a large area (mm range) of reproducible submicron pillar-based topographies. Using the two-photon polymerization (2PP) as a 3D printing technique based on direct laser writing, uniform submicron patterns were created through optimization of the process parameters and writing strategy. To demonstrate the multiscale fabrication capabilities of this approach, submicron pillars of various heights were integrated onto the surfaces of a 3D microscaffold in a single-step 2PP process. The created submicron topography was also found to improve the hydrophilicity of the surface while being able to withstand flow rates of up to 8 mL/min. The material (IP-Dip resin) used for patterning did not have cytotoxic effects against human mesenchymal stromal cells after 3 days of dynamic culture in the microfluidic device. This proof-of-principle study, therefore, marks a significant step forward in manufacturing submicron structure-on-a-chip models for bone regeneration studies.

AB - Manufacturing high throughput in vitro models resembling the tissue microenvironment is highly demanded for studying bone regeneration. Tissues such as bone have complex multiscale architectures inside which cells reside. To this end, engineering a microfluidic platform incorporated with three-dimensional (3D) microscaffolds and submicron/nanoscale topographies can provide a promising model for 3D cell cultures. There are, however, certain challenges associated with this goal, such as the need to decorate large surfaces area with high-fidelity 3D submicron structures. Here, we succeeded in fabricating a microfluidic platform embedded with a large area (mm range) of reproducible submicron pillar-based topographies. Using the two-photon polymerization (2PP) as a 3D printing technique based on direct laser writing, uniform submicron patterns were created through optimization of the process parameters and writing strategy. To demonstrate the multiscale fabrication capabilities of this approach, submicron pillars of various heights were integrated onto the surfaces of a 3D microscaffold in a single-step 2PP process. The created submicron topography was also found to improve the hydrophilicity of the surface while being able to withstand flow rates of up to 8 mL/min. The material (IP-Dip resin) used for patterning did not have cytotoxic effects against human mesenchymal stromal cells after 3 days of dynamic culture in the microfluidic device. This proof-of-principle study, therefore, marks a significant step forward in manufacturing submicron structure-on-a-chip models for bone regeneration studies.

KW - bone regeneration

KW - microfluidics

KW - submicron pillars

KW - two-photon polymerization

UR - http://www.scopus.com/inward/record.url?scp=85073693818&partnerID=8YFLogxK

U2 - 10.1021/acsbiomaterials.9b01155

DO - 10.1021/acsbiomaterials.9b01155

M3 - Article

VL - 5

SP - 6127

EP - 6136

JO - ACS Biomaterials Science and Engineering

JF - ACS Biomaterials Science and Engineering

SN - 2373-9878

IS - 11

ER -

ID: 62688549