Abstract
During transcription RNA polymerase (RNAP) moves along a DNA molecule to copy the information on the DNA to an RNA molecule. Many textbook pictures show an RNAP sliding along empty DNA, but in reality it is crowded on the DNA and RNAP competes for space with many proteins such as other RNAP’s and histones. Coverage of DNA by histones is essential for DNA protection and signaling. However, during transcription RNAP evicts histones, which then rebind quickly or are replaced by other proteins. How does crowding of RNAP and histones on the DNA affects transcription dynamics on the one hand, and how does transcription activity change the density and exchange of histones along the DNA on the other hand? Those are the central questions of this thesis.
Original language | English |
---|---|
Awarding Institution |
|
Supervisors/Advisors |
|
Thesis sponsors | |
Award date | 29 Sept 2017 |
Print ISBNs | 978-90-8593-308-3 |
DOIs | |
Publication status | Published - 2017 |
Bibliographical note
Casimir PhD series, Delft-Leiden 2017-24Keywords
- Transcription
- transcriptional bursts
- TASEP
- Crowding
- Nucleosomes
- Bus route model