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The progression of replication forks at natural replication barriers in live bacteria. / Moolman, M. Charl; Tiruvadi Krishnan, S; Kerssemakers, Jacob W J; De Leeuw, Roy; Lorent, Vincent; Sherratt, David J.; Dekker, Nynke H.

In: Nucleic Acids Research, Vol. 44, No. 13, 2016, p. 6262-6273.

Research output: Contribution to journalArticleScientificpeer-review

Harvard

Moolman, MC, Tiruvadi Krishnan, S, Kerssemakers, JWJ, De Leeuw, R, Lorent, V, Sherratt, DJ & Dekker, NH 2016, 'The progression of replication forks at natural replication barriers in live bacteria', Nucleic Acids Research, vol. 44, no. 13, pp. 6262-6273. https://doi.org/10.1093/nar/gkw397

APA

Moolman, M. C., Tiruvadi Krishnan, S., Kerssemakers, J. W. J., De Leeuw, R., Lorent, V., Sherratt, D. J., & Dekker, N. H. (2016). The progression of replication forks at natural replication barriers in live bacteria. Nucleic Acids Research, 44(13), 6262-6273. https://doi.org/10.1093/nar/gkw397

Vancouver

Moolman MC, Tiruvadi Krishnan S, Kerssemakers JWJ, De Leeuw R, Lorent V, Sherratt DJ et al. The progression of replication forks at natural replication barriers in live bacteria. Nucleic Acids Research. 2016;44(13):6262-6273. https://doi.org/10.1093/nar/gkw397

Author

Moolman, M. Charl ; Tiruvadi Krishnan, S ; Kerssemakers, Jacob W J ; De Leeuw, Roy ; Lorent, Vincent ; Sherratt, David J. ; Dekker, Nynke H. / The progression of replication forks at natural replication barriers in live bacteria. In: Nucleic Acids Research. 2016 ; Vol. 44, No. 13. pp. 6262-6273.

BibTeX

@article{64c5ef9cde664098a5f839c3aed08a5b,
title = "The progression of replication forks at natural replication barriers in live bacteria",
abstract = "Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood. By performing quantitative fluorescence microscopy with microfuidics, we investigate the effect on the replisome when encountering these barriers in live Escherichia coli cells. We make use of an E. coli variant that includes only an ectopic origin of replication that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other replisome. This enables us to single out the effect of encountering a Tus-ter roadblock on an individual replisome. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. These observations demonstrate the resilience of the replication fork to natural barriers and the sensitivity of chromosome alignment to fork progression.",
keywords = "Genome integrity, repair and replication",
author = "Moolman, {M. Charl} and {Tiruvadi Krishnan}, S and Kerssemakers, {Jacob W J} and {De Leeuw}, Roy and Vincent Lorent and Sherratt, {David J.} and Dekker, {Nynke H.}",
year = "2016",
doi = "10.1093/nar/gkw397",
language = "English",
volume = "44",
pages = "6262--6273",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",

}

RIS

TY - JOUR

T1 - The progression of replication forks at natural replication barriers in live bacteria

AU - Moolman, M. Charl

AU - Tiruvadi Krishnan, S

AU - Kerssemakers, Jacob W J

AU - De Leeuw, Roy

AU - Lorent, Vincent

AU - Sherratt, David J.

AU - Dekker, Nynke H.

PY - 2016

Y1 - 2016

N2 - Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood. By performing quantitative fluorescence microscopy with microfuidics, we investigate the effect on the replisome when encountering these barriers in live Escherichia coli cells. We make use of an E. coli variant that includes only an ectopic origin of replication that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other replisome. This enables us to single out the effect of encountering a Tus-ter roadblock on an individual replisome. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. These observations demonstrate the resilience of the replication fork to natural barriers and the sensitivity of chromosome alignment to fork progression.

AB - Protein-DNA complexes are one of the principal barriers the replisome encounters during replication. One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression. The details concerning the dynamics of the replisome when encountering these Tus-ter barriers in the cell are poorly understood. By performing quantitative fluorescence microscopy with microfuidics, we investigate the effect on the replisome when encountering these barriers in live Escherichia coli cells. We make use of an E. coli variant that includes only an ectopic origin of replication that is positioned such that one of the two replisomes encounters a Tus-ter barrier before the other replisome. This enables us to single out the effect of encountering a Tus-ter roadblock on an individual replisome. We demonstrate that the replisome remains stably bound after encountering a Tus-ter complex from the non-permissive direction. Furthermore, the replisome is only transiently blocked, and continues replication beyond the barrier. Additionally, we demonstrate that these barriers affect sister chromosome segregation by visualizing specific chromosomal loci in the presence and absence of the Tus protein. These observations demonstrate the resilience of the replication fork to natural barriers and the sensitivity of chromosome alignment to fork progression.

KW - Genome integrity

KW - repair and replication

UR - http://www.scopus.com/inward/record.url?scp=84982074338&partnerID=8YFLogxK

UR - http://resolver.tudelft.nl/uuid:64c5ef9c-de66-4098-a5f8-39c3aed08a5b

U2 - 10.1093/nar/gkw397

DO - 10.1093/nar/gkw397

M3 - Article

AN - SCOPUS:84982074338

VL - 44

SP - 6262

EP - 6273

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 13

ER -

ID: 7335974