TY - JOUR
T1 - Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media
AU - Fernández-Fueyo, Elena
AU - Ni, Yan
AU - Gomez Baraibar, Alvaro
AU - Alcalde, Miguel
AU - van Langen, Lukas M.
AU - Hollmann, Frank
PY - 2016
Y1 - 2016
N2 - The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant. Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90,000 turnovers of the biocatalyst.
AB - The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant. Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90,000 turnovers of the biocatalyst.
KW - Biocatalysis
KW - Hydroxylation
KW - Non-aqueous reaction media
KW - Oxyfunctionalization
KW - Peroxygenase
UR - http://resolver.tudelft.nl/uuid:b79f43a7-febf-4982-b68f-decc63acc5d2
UR - http://www.scopus.com/inward/record.url?scp=84994518192&partnerID=8YFLogxK
U2 - 10.1016/j.molcatb.2016.09.013
DO - 10.1016/j.molcatb.2016.09.013
M3 - Article
SN - 1381-1177
VL - 134
SP - 347
EP - 352
JO - Journal of Molecular Catalysis B: Enzymatic
JF - Journal of Molecular Catalysis B: Enzymatic
ER -