Why Argonaute is needed to make microRNA target search fast and reliable

Misha Klein; Stanley D. Chandradoss; Martin Depken; Chirlmin Joo

doi:10.1016/j.semcdb.2016.05.017

Why Argonaute is needed to make microRNA target search fast and reliable

Misha Klein, Stanley D. Chandradoss, Martin Depken^*, Chirlmin Joo

^*Corresponding author for this work

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Abstract

MicroRNA (miRNA) interferes with the translation of cognate messenger RNA (mRNA) by finding, preferentially binding, and marking it for degradation. To facilitate the search process, Argonaute (Ago) proteins come together with miRNA, forming a dynamic search complex. In this review we use the language of free-energy landscapes to discuss recent single-molecule and high-resolution structural data in the light of theoretical work appropriated from the study of transcription-factor search. We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.

Original language	English
Pages (from-to)	20-28
Journal	Seminars in Cell and Developmental Biology
Volume	65
DOIs	https://doi.org/10.1016/j.semcdb.2016.05.017
Publication status	Published - 2017

Keywords

Argonaute
Free-energy landscape
FRET
Lateral diffusion
MicroRNA target prediction
MiRNA
RNA silencing
Search stability paradox
Single molecule
Target Search
Theoretical model

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10.1016/j.semcdb.2016.05.017

1-s2.0-S1084952116301434-mainFinal published version, 1.82 MBLicence: CC BY-NC-ND

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title = "Why Argonaute is needed to make microRNA target search fast and reliable",

abstract = "MicroRNA (miRNA) interferes with the translation of cognate messenger RNA (mRNA) by finding, preferentially binding, and marking it for degradation. To facilitate the search process, Argonaute (Ago) proteins come together with miRNA, forming a dynamic search complex. In this review we use the language of free-energy landscapes to discuss recent single-molecule and high-resolution structural data in the light of theoretical work appropriated from the study of transcription-factor search. We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.",

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AU - Klein, Misha

AU - Chandradoss, Stanley D.

AU - Depken, Martin

AU - Joo, Chirlmin

PY - 2017

Y1 - 2017

N2 - MicroRNA (miRNA) interferes with the translation of cognate messenger RNA (mRNA) by finding, preferentially binding, and marking it for degradation. To facilitate the search process, Argonaute (Ago) proteins come together with miRNA, forming a dynamic search complex. In this review we use the language of free-energy landscapes to discuss recent single-molecule and high-resolution structural data in the light of theoretical work appropriated from the study of transcription-factor search. We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.

AB - MicroRNA (miRNA) interferes with the translation of cognate messenger RNA (mRNA) by finding, preferentially binding, and marking it for degradation. To facilitate the search process, Argonaute (Ago) proteins come together with miRNA, forming a dynamic search complex. In this review we use the language of free-energy landscapes to discuss recent single-molecule and high-resolution structural data in the light of theoretical work appropriated from the study of transcription-factor search. We suggest that experimentally observed internal states of the Ago-miRNA search complex may have the explicit biological function of speeding up search while maintaining specificity.

KW - Argonaute

KW - Free-energy landscape

KW - FRET

KW - Lateral diffusion

KW - MicroRNA target prediction

KW - MiRNA

KW - RNA silencing

KW - Search stability paradox

KW - Single molecule

KW - Target Search

KW - Theoretical model

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JO - Seminars in Cell and Developmental Biology

JF - Seminars in Cell and Developmental Biology

ER -

Why Argonaute is needed to make microRNA target search fast and reliable

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Keywords

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